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2l3e
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| - | [[ | + | ==Solution structure of P2a-J2a/b-P2b of human telomerase RNA== |
| + | <StructureSection load='2l3e' size='340' side='right' caption='[[2l3e]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2l3e]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L3E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L3E FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l3e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l3e RCSB], [http://www.ebi.ac.uk/pdbsum/2l3e PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Telomerase is a unique ribonucleoprotein complex that catalyzes the addition of telomeric DNA repeats onto the 3' ends of linear chromosomes. All vertebrate telomerase RNAs contain a catalytically essential core domain that includes the template and a pseudoknot with extended helical subdomains. Within these helical regions is an asymmetric 5-nt internal bulge loop (J2a/b) flanked by helices (P2a and P2b) that is highly conserved in its location but not sequence. NMR structure determination reveals that J2a/b forms a defined S-shape and creates an approximately 90 degrees bend with a surprisingly low twist ( approximately 10 degrees ) between the flanking helices. A search of RNA structures revealed only one other example of a 5-nt bulge, from hepatitis C virus internal ribosome entry site, with a different sequence but the same structure. J2a/b is intrinsically flexible but the interhelical motions across the loop are remarkably restricted. Nucleotide substitutions in J2a/b that affect the bend angle, direction, and interhelical dynamics are correlated with telomerase activity. Based on the structures of P2ab (J2a/b and flanking helices), the conserved region of the pseudoknot (P2b/P3, previously determined) and the remaining helical segment (P2a.1-J2a.1 refined using residual dipolar couplings and the modeling program MC-Sym) we have calculated an NMR-based model of the full-length pseudoknot. The model and dynamics analysis show that J2a/b serves as a dominant structural and dynamical element in defining the overall topology of the core domain, and suggest that interhelical motions in P2ab facilitate nucleotide addition along the template and template translocation. | ||
| - | + | Structurally conserved five nucleotide bulge determines the overall topology of the core domain of human telomerase RNA.,Zhang Q, Kim NK, Peterson RD, Wang Z, Feigon J Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):18761-8. Epub 2010 Oct 21. PMID:20966348<ref>PMID:20966348</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | + | ||
| - | == | + | |
| - | < | + | |
[[Category: Feigon, J.]] | [[Category: Feigon, J.]] | ||
[[Category: Kim, N.]] | [[Category: Kim, N.]] | ||
Revision as of 11:33, 20 October 2014
Solution structure of P2a-J2a/b-P2b of human telomerase RNA
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Categories: Feigon, J. | Kim, N. | Peterson, R D. | Wang, Z. | Zhang, Q. | Htr | Human telomerase rna | J2ab | P2a | P2b | Rna
