2y0r
From Proteopedia
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| - | [[ | + | ==STRUCTURAL BASIS FOR THE ALLOSTERIC INTERFERENCE OF MYOSIN FUNCTION BY MUTANTS G680A AND G680V OF DICTYOSTELIUM MYOSIN-2== |
| + | <StructureSection load='2y0r' size='340' side='right' caption='[[2y0r]], [[Resolution|resolution]] 2.85Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2y0r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y0R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Y0R FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1mne|1mne]], [[1d1c|1d1c]], [[1jx2|1jx2]], [[1q5g|1q5g]], [[1w9l|1w9l]], [[1vom|1vom]], [[1d0y|1d0y]], [[1mmn|1mmn]], [[2aka|2aka]], [[2jhr|2jhr]], [[2jj9|2jj9]], [[2xel|2xel]], [[1lvk|1lvk]], [[1w9j|1w9j]], [[1jwy|1jwy]], [[2x9h|2x9h]], [[1fmv|1fmv]], [[1yv3|1yv3]], [[1d0x|1d0x]], [[1fmw|1fmw]], [[1d1b|1d1b]], [[1g8x|1g8x]], [[1mma|1mma]], [[1d1a|1d1a]], [[1mmg|1mmg]], [[2xo8|2xo8]], [[1w9i|1w9i]], [[1d0z|1d0z]], [[1mnd|1mnd]], [[1w9k|1w9k]], [[1mmd|1mmd]], [[2y8i|2y8i]], [[2y9e|2y9e]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y0r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2y0r RCSB], [http://www.ebi.ac.uk/pdbsum/2y0r PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The cold-sensitive single-residue mutation of glycine 680 in the reactive thiol region of Dictyostelium discoideum myosin-2 or the corresponding conserved glycine in other myosin isoforms has been reported to interfere with motor function. Here we present the x-ray structures of myosin motor domain mutants G680A in the absence and presence of nucleotide as well as the apo structure of mutant G680V. Our results show that the Gly-680 mutations lead to uncoupling of the reactive thiol region from the surrounding structural elements. Structural and functional data indicate that the mutations induce the preferential population of a state that resembles the ADP-bound state. Moreover, the Gly-680 mutants display greatly reduced dynamic properties, which appear to be related to the recovery of myosin motor function at elevated temperatures. | ||
| - | + | Structural Basis for the Allosteric Interference of Myosin Function by Reactive Thiol Region Mutations G680A and G680V.,Preller M, Bauer S, Adamek N, Fujita-Becker S, Fedorov R, Geeves MA, Manstein DJ J Biol Chem. 2011 Oct 7;286(40):35051-60. Epub 2011 Aug 13. PMID:21841195<ref>PMID:21841195</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Myosin|Myosin]] | *[[Myosin|Myosin]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Dictyostelium discoideum]] | [[Category: Dictyostelium discoideum]] | ||
[[Category: Adamek, N.]] | [[Category: Adamek, N.]] | ||
Revision as of 12:53, 22 October 2014
STRUCTURAL BASIS FOR THE ALLOSTERIC INTERFERENCE OF MYOSIN FUNCTION BY MUTANTS G680A AND G680V OF DICTYOSTELIUM MYOSIN-2
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