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2xlk

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[[Image:2xlk.png|left|200px]]
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==CRYSTAL STRUCTURE OF THE CSY4-CRRNA COMPLEX, ORTHORHOMBIC FORM==
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<StructureSection load='2xlk' size='340' side='right' caption='[[2xlk]], [[Resolution|resolution]] 1.81&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2xlk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XLK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XLK FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xli|2xli]], [[2xlj|2xlj]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xlk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xlk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xlk RCSB], [http://www.ebi.ac.uk/pdbsum/2xlk PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xl/2xlk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Many bacteria and archaea contain clustered regularly interspaced short palindromic repeats (CRISPRs) that confer resistance to invasive genetic elements. Central to this immune system is the production of CRISPR-derived RNAs (crRNAs) after transcription of the CRISPR locus. Here, we identify the endoribonuclease (Csy4) responsible for CRISPR transcript (pre-crRNA) processing in Pseudomonas aeruginosa. A 1.8 angstrom crystal structure of Csy4 bound to its cognate RNA reveals that Csy4 makes sequence-specific interactions in the major groove of the crRNA repeat stem-loop. Together with electrostatic contacts to the phosphate backbone, these enable Csy4 to bind selectively and cleave pre-crRNAs using phylogenetically conserved serine and histidine residues in the active site. The RNA recognition mechanism identified here explains sequence- and structure-specific processing by a large family of CRISPR-specific endoribonucleases.
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{{STRUCTURE_2xlk| PDB=2xlk | SCENE= }}
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Sequence- and structure-specific RNA processing by a CRISPR endonuclease.,Haurwitz RE, Jinek M, Wiedenheft B, Zhou K, Doudna JA Science. 2010 Sep 10;329(5997):1355-8. PMID:20829488<ref>PMID:20829488</ref>
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===CRYSTAL STRUCTURE OF THE CSY4-CRRNA COMPLEX, ORTHORHOMBIC FORM===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_20829488}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2xlk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XLK OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:020829488</ref><references group="xtra"/>
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[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Doudna, J A.]]
[[Category: Doudna, J A.]]

Revision as of 13:02, 22 October 2014

CRYSTAL STRUCTURE OF THE CSY4-CRRNA COMPLEX, ORTHORHOMBIC FORM

2xlk, resolution 1.81Å

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