1k8d
From Proteopedia
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| - | [[Image:1k8d.jpg|left|200px]] | + | [[Image:1k8d.jpg|left|200px]] |
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| - | '''crystal structure of the non-classical MHC class Ib Qa-2 complexed with a self peptide''' | + | {{Structure |
| + | |PDB= 1k8d |SIZE=350|CAPTION= <scene name='initialview01'>1k8d</scene>, resolution 2.30Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= Q9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | ||
| + | }} | ||
| + | |||
| + | '''crystal structure of the non-classical MHC class Ib Qa-2 complexed with a self peptide''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1K8D is a [ | + | 1K8D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K8D OCA]. |
==Reference== | ==Reference== | ||
| - | Promiscuous antigen presentation by the nonclassical MHC Ib Qa-2 is enabled by a shallow, hydrophobic groove and self-stabilized peptide conformation., He X, Tabaczewski P, Ho J, Stroynowski I, Garcia KC, Structure. 2001 Dec;9(12):1213-24. PMID:[http:// | + | Promiscuous antigen presentation by the nonclassical MHC Ib Qa-2 is enabled by a shallow, hydrophobic groove and self-stabilized peptide conformation., He X, Tabaczewski P, Ho J, Stroynowski I, Garcia KC, Structure. 2001 Dec;9(12):1213-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11738047 11738047] |
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: qa-2]] | [[Category: qa-2]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:13:52 2008'' |
Revision as of 10:13, 20 March 2008
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| , resolution 2.30Å | |||||||
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| Gene: | Q9 (Mus musculus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
crystal structure of the non-classical MHC class Ib Qa-2 complexed with a self peptide
Overview
BACKGROUND: Qa-2 is a nonclassical MHC Ib antigen, which has been implicated in both innate and adaptive immune responses, as well as embryonic development. Qa-2 has an unusual peptide binding specificity in that it requires two dominant C-terminal anchor residues and is capable of associating with a substantially more diverse array of peptide sequences than other nonclassical MHC. RESULTS: We have determined the crystal structure, to 2.3 A, of the Q9 gene of murine Qa-2 complexed with a self-peptide derived from the L19 ribosomal protein, which is abundant in the pool of peptides eluted from the Q9 groove. The 9 amino acid peptide is bound high in a shallow, hydrophobic binding groove of Q9, which is missing a C pocket. The peptide makes few specific contacts and exhibits extremely poor shape complementarity to the MHC groove, which facilitates the presentation of a degenerate array of sequences. The L19 peptide is in a centrally bulged conformation that is stabilized by intramolecular interactions from the invariant P7 histidine anchor residue and by a hydrophobic core of preferred secondary anchor residues that have minimal interaction with the MHC. CONCLUSIONS: Unexpectedly, the preferred secondary peptide residues that exhibit tenuous contact with Q9 contribute significantly to the overall stability of the peptide-MHC complex. The structure of this complex implies a "conformational" selection by Q9 for peptide residues that optimally stabilize the large bulge rather than making intimate contact with the MHC pockets.
About this Structure
1K8D is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Promiscuous antigen presentation by the nonclassical MHC Ib Qa-2 is enabled by a shallow, hydrophobic groove and self-stabilized peptide conformation., He X, Tabaczewski P, Ho J, Stroynowski I, Garcia KC, Structure. 2001 Dec;9(12):1213-24. PMID:11738047
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