1kdg

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[[Image:1kdg.gif|left|200px]]<br /><applet load="1kdg" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1kdg.gif|left|200px]]
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caption="1kdg, resolution 1.50&Aring;" />
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'''Crystal structure of the flavin domain of cellobiose dehydrogenase'''<br />
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{{Structure
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|PDB= 1kdg |SIZE=350|CAPTION= <scene name='initialview01'>1kdg</scene>, resolution 1.50&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=6FA:6-HYDROXY-FLAVIN-ADENINE+DINUCLEOTIDE'>6FA</scene>, <scene name='pdbligand=EMT:2-(ETHYLMERCURI-THIO)-BENZOIC+ACID'>EMT</scene> and <scene name='pdbligand=S:SULFUR ATOM'>S</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Crystal structure of the flavin domain of cellobiose dehydrogenase'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1KDG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=MAN:'>MAN</scene>, <scene name='pdbligand=HG:'>HG</scene>, <scene name='pdbligand=6FA:'>6FA</scene>, <scene name='pdbligand=EMT:'>EMT</scene> and <scene name='pdbligand=S:'>S</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KDG OCA].
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1KDG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KDG OCA].
==Reference==
==Reference==
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Crystal structure of the flavoprotein domain of the extracellular flavocytochrome cellobiose dehydrogenase., Hallberg BM, Henriksson G, Pettersson G, Divne C, J Mol Biol. 2002 Jan 18;315(3):421-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11786022 11786022]
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Crystal structure of the flavoprotein domain of the extracellular flavocytochrome cellobiose dehydrogenase., Hallberg BM, Henriksson G, Pettersson G, Divne C, J Mol Biol. 2002 Jan 18;315(3):421-34. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11786022 11786022]
[[Category: Phanerochaete chrysosporium]]
[[Category: Phanerochaete chrysosporium]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: rossmann fold]]
[[Category: rossmann fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:32:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:15:53 2008''

Revision as of 10:15, 20 March 2008

Image:1kdg.gif


PDB ID 1kdg

Drag the structure with the mouse to rotate
, resolution 1.50Å
Ligands: , , , , and
Coordinates: save as pdb, mmCIF, xml



Crystal structure of the flavin domain of cellobiose dehydrogenase


Overview

Cellobiose dehydrogenase (CDH) participates in the degradation of cellulose and lignin. The protein is an extracellular flavocytochrome with a b-type cytochrome domain (CYT(cdh)) connected to a flavodehydrogenase domain (DH(cdh)). DH(cdh) catalyses a two-electron oxidation at the anomeric C1 position of cellobiose to yield cellobiono-1,5-lactone, and the electrons are subsequently transferred from DH(cdh) to an acceptor, either directly or via CYT(cdh). Here, we describe the crystal structure of Phanerochaete chrysosporium DH(cdh) determined at 1.5 A resolution. DH(cdh) belongs to the GMC family of oxidoreductases, which includes glucose oxidase (GOX) and cholesterol oxidase (COX); however, the sequence identity with members of the family is low. The overall fold of DH(cdh) is p-hydroxybenzoate hydroxylase-like and is similar to, but also different from, that of GOX and COX. It is partitioned into an FAD-binding subdomain of alpha/beta type and a substrate-binding subdomain consisting of a seven-stranded beta sheet and six helices. Docking of CYT(cdh) and DH(cdh) suggests that CYT(cdh) covers the active-site entrance in DH(cdh), and that the resulting distance between the cofactors is within acceptable limits for inter-domain electron transfer. Based on docking of the substrate, cellobiose, in the active site of DH(cdh), we propose that the enzyme discriminates against glucose by favouring interaction with the non-reducing end of cellobiose.

About this Structure

1KDG is a Single protein structure of sequence from Phanerochaete chrysosporium. Full crystallographic information is available from OCA.

Reference

Crystal structure of the flavoprotein domain of the extracellular flavocytochrome cellobiose dehydrogenase., Hallberg BM, Henriksson G, Pettersson G, Divne C, J Mol Biol. 2002 Jan 18;315(3):421-34. PMID:11786022

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