1khq

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1khq.gif|left|200px]]<br /><applet load="1khq" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1khq.gif|left|200px]]
-
caption="1khq, resolution 1.6&Aring;" />
+
 
-
'''ORTHORHOMBIC FORM OF PAPAIN/ZLFG-DAM COVALENT COMPLEX'''<br />
+
{{Structure
 +
|PDB= 1khq |SIZE=350|CAPTION= <scene name='initialview01'>1khq</scene>, resolution 1.6&Aring;
 +
|SITE=
 +
|LIGAND=
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/Papain Papain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.2 3.4.22.2]
 +
|GENE=
 +
}}
 +
 
 +
'''ORTHORHOMBIC FORM OF PAPAIN/ZLFG-DAM COVALENT COMPLEX'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1KHQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Carica_papaya Carica papaya]. Active as [http://en.wikipedia.org/wiki/Papain Papain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.2 3.4.22.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHQ OCA].
+
1KHQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Carica_papaya Carica papaya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHQ OCA].
==Reference==
==Reference==
-
Two polymorphs of a covalent complex between papain and a diazomethylketone inhibitor., Janowski R, Kozak M, Jankowska E, Grzonka Z, Jaskolski M, J Pept Res. 2004 Oct;64(4):141-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15357669 15357669]
+
Two polymorphs of a covalent complex between papain and a diazomethylketone inhibitor., Janowski R, Kozak M, Jankowska E, Grzonka Z, Jaskolski M, J Pept Res. 2004 Oct;64(4):141-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15357669 15357669]
[[Category: Carica papaya]]
[[Category: Carica papaya]]
[[Category: Papain]]
[[Category: Papain]]
Line 23: Line 32:
[[Category: protease inhibitor]]
[[Category: protease inhibitor]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:34:18 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:17:37 2008''

Revision as of 10:17, 20 March 2008

Image:1khq.gif


PDB ID 1khq

Drag the structure with the mouse to rotate
, resolution 1.6Å
Activity: Papain, with EC number 3.4.22.2
Coordinates: save as pdb, mmCIF, xml



ORTHORHOMBIC FORM OF PAPAIN/ZLFG-DAM COVALENT COMPLEX


Overview

The three-dimensional structure of two polymorphs of a ZLFG-CH2-papain covalent complex has been determined by X-ray crystallography. The structures indicate that: (i) the methylene carbon atom of the inhibitor is covalently bound to the Sgamma atom of Cys25 of papain; (ii) the hydrophobic S2 pocket formed by Pro68, Val133, Val157, and Asp158 is occupied by the inhibitor's phenylalanyl P2 side chain; (iii) extensive hydrogen bonding and hydrophobic interactions are responsible for the interaction of the inhibitor with the enzyme. Comparison with similar structures suggests that in covalent complexes preservation of main chain-main chain interactions between the enzyme and the inhibitor may have higher priority than the P-S interactions.

About this Structure

1KHQ is a Single protein structure of sequence from Carica papaya. Full crystallographic information is available from OCA.

Reference

Two polymorphs of a covalent complex between papain and a diazomethylketone inhibitor., Janowski R, Kozak M, Jankowska E, Grzonka Z, Jaskolski M, J Pept Res. 2004 Oct;64(4):141-50. PMID:15357669

Page seeded by OCA on Thu Mar 20 12:17:37 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1khq"
Personal tools