1l1c
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1l1c.gif|left|200px]] | + | [[Image:1l1c.gif|left|200px]] |
| - | + | ||
| - | '''Structure of the LicT Bacterial Antiterminator Protein in Complex with its RNA Target''' | + | {{Structure |
| + | |PDB= 1l1c |SIZE=350|CAPTION= <scene name='initialview01'>1l1c</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= LicT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of the LicT Bacterial Antiterminator Protein in Complex with its RNA Target''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1L1C is a [ | + | 1L1C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L1C OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure of the LicT-RNA antitermination complex: CAT clamping RAT., Yang Y, Declerck N, Manival X, Aymerich S, Kochoyan M, EMBO J. 2002 Apr 15;21(8):1987-97. PMID:[http:// | + | Solution structure of the LicT-RNA antitermination complex: CAT clamping RAT., Yang Y, Declerck N, Manival X, Aymerich S, Kochoyan M, EMBO J. 2002 Apr 15;21(8):1987-97. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11953318 11953318] |
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 22: | Line 31: | ||
[[Category: rna hairpin]] | [[Category: rna hairpin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:24:59 2008'' |
Revision as of 10:25, 20 March 2008
| |||||||
| Gene: | LicT (Bacillus subtilis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the LicT Bacterial Antiterminator Protein in Complex with its RNA Target
Overview
LicT is a bacterial regulatory protein able to prevent the premature arrest of transcription. When activated, LicT binds to a 29 base RNA hairpin overlapping a terminator located in the 5' mRNA leader region of the target genes. We have determined the solution structure of the LicT RNA-binding domain (CAT) in complex with its ribonucleic antiterminator (RAT) target by NMR spectroscopy (PDB 1L1C). CAT is a beta-stranded homodimer that undergoes no important conformational changes upon complex formation. It interacts, through mostly hydrophobic and stacking interactions, with the distorted minor groove of the hairpin stem that is interrupted by two asymmetric internal loops. Although different in sequence, these loops share sufficient structural analogy to be recognized similarly by symmetry-related elements of the protein dimer, leading to a quasi- symmetric structure reminiscent of that observed with dimeric transcription regulators bound to palindromic DNA. Sequence analysis suggests that this RNA- binding mode, where the RAT strands are clamped by the CAT dimer, is conserved in homologous systems.
About this Structure
1L1C is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Solution structure of the LicT-RNA antitermination complex: CAT clamping RAT., Yang Y, Declerck N, Manival X, Aymerich S, Kochoyan M, EMBO J. 2002 Apr 15;21(8):1987-97. PMID:11953318
Page seeded by OCA on Thu Mar 20 12:24:59 2008
