1l5h

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[[Image:1l5h.jpg|left|200px]]<br /><applet load="1l5h" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1l5h.jpg|left|200px]]
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caption="1l5h, resolution 2.3&Aring;" />
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'''FeMo-cofactor Deficient Nitrogenase MoFe Protein'''<br />
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{{Structure
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|PDB= 1l5h |SIZE=350|CAPTION= <scene name='initialview01'>1l5h</scene>, resolution 2.3&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=CLF:FE(8)-S(7) CLUSTER'>CLF</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Nitrogenase Nitrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.6.1 1.18.6.1]
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|GENE=
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}}
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'''FeMo-cofactor Deficient Nitrogenase MoFe Protein'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1L5H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=CLF:'>CLF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrogenase Nitrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.6.1 1.18.6.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5H OCA].
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1L5H is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5H OCA].
==Reference==
==Reference==
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Structure of a cofactor-deficient nitrogenase MoFe protein., Schmid B, Ribbe MW, Einsle O, Yoshida M, Thomas LM, Dean DR, Rees DC, Burgess BK, Science. 2002 Apr 12;296(5566):352-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11951047 11951047]
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Structure of a cofactor-deficient nitrogenase MoFe protein., Schmid B, Ribbe MW, Einsle O, Yoshida M, Thomas LM, Dean DR, Rees DC, Burgess BK, Science. 2002 Apr 12;296(5566):352-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11951047 11951047]
[[Category: Azotobacter vinelandii]]
[[Category: Azotobacter vinelandii]]
[[Category: Nitrogenase]]
[[Category: Nitrogenase]]
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[[Category: apo-protein]]
[[Category: apo-protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:41:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:26:36 2008''

Revision as of 10:26, 20 March 2008

Image:1l5h.jpg


PDB ID 1l5h

Drag the structure with the mouse to rotate
, resolution 2.3Å
Ligands: and
Activity: Nitrogenase, with EC number 1.18.6.1
Coordinates: save as pdb, mmCIF, xml



FeMo-cofactor Deficient Nitrogenase MoFe Protein


Overview

One of the most complex biosynthetic processes in metallobiochemistry is the assembly of nitrogenase, the key enzyme in biological nitrogen fixation. We describe here the crystal structure of an iron-molybdenum cofactor-deficient form of the nitrogenase MoFe protein, into which the cofactor is inserted in the final step of MoFe protein assembly. The MoFe protein folds as a heterotetramer containing two copies each of the homologous alpha and beta subunits. In this structure, one of the three alpha subunit domains exhibits a substantially changed conformation, whereas the rest of the protein remains essentially unchanged. A predominantly positively charged funnel is revealed; this funnel is of sufficient size to accommodate insertion of the negatively charged cofactor.

About this Structure

1L5H is a Protein complex structure of sequences from Azotobacter vinelandii. Full crystallographic information is available from OCA.

Reference

Structure of a cofactor-deficient nitrogenase MoFe protein., Schmid B, Ribbe MW, Einsle O, Yoshida M, Thomas LM, Dean DR, Rees DC, Burgess BK, Science. 2002 Apr 12;296(5566):352-6. PMID:11951047

Page seeded by OCA on Thu Mar 20 12:26:36 2008

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