This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
3a3h
From Proteopedia
| Line 25: | Line 25: | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 17:44:42 2007'' |
Revision as of 15:39, 30 October 2007
|
CELLOTRIOSE COMPLEX OF THE ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHERANS AT 1.6 A RESOLUTION
Overview
The enzymatic hydrolysis of O-glycosidic linkages is one of the most, diverse and widespread reactions in nature and involves a classic, "textbook" enzyme mechanism. A multidisciplinary analysis of a, beta-glycoside hydrolase, the Cel5A from Bacillus agaradhaerens, is, presented in which the structures of each of the native, substrate, covalent-intermediate, and product complexes have been determined and, their interconversions analyzed kinetically, providing unprecedented, insights into the mechanism of this enzyme class. Substrate is bound in a, distorted 1S3 skew-boat conformation, thereby presenting the anomeric, carbon appropriately for nucleophilic attack as well as satisfying the, stereoelectronic requirements for an incipient oxocarbenium ion. Leaving, group departure results in ... [(full description)]
About this Structure
3A3H is a [Single protein] structure of sequence from [Bacillus agaradhaerens] with CTR as [ligand]. Active as [Cellulase], with EC number [3.2.1.4]. Structure known Active Sites: ACI and NUC. Full crystallographic information is available from [OCA].
Reference
Snapshots along an enzymatic reaction coordinate: analysis of a retaining beta-glycoside hydrolase., Davies GJ, Mackenzie L, Varrot A, Dauter M, Brzozowski AM, Schulein M, Withers SG, Biochemistry. 1998 Aug 25;37(34):11707-13. PMID:9718293
Page seeded by OCA on Tue Oct 30 17:44:42 2007
