Cellulosomal scaffolding protein
From Proteopedia
(Difference between revisions)
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| - | == Carbohydrate Binding Module (Family 3 | + | == Carbohydrate Binding Module (Family 3 Clostridium Thermicellum)== |
<StructureSection load='4JO5' size='340' side='right' caption='Caption for this structure' scene=''> | <StructureSection load='4JO5' size='340' side='right' caption='Caption for this structure' scene=''> | ||
| - | + | Carbohydrate-binding domain modules(CBM) are non-catalytic domain which present in various microrganisms. <ref>doi:10.1016/j.pep.2008.01.018</ref> | |
| - | + | ||
== Function == | == Function == | ||
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<scene name='60/609843/Beta_sheets/1'>Beta Sheets</scene> | <scene name='60/609843/Beta_sheets/1'>Beta Sheets</scene> | ||
| - | == | + | == Applications == |
| - | + | The cellulose-binding module (CBM) is an attractive affinity tag for protein purification for several reasons: | |
| + | 1.Very specific binding ability to the fused protein eith CBM tag. | ||
| + | 2.Very low nonspecific binding ability to to other protein. | ||
| + | 3.efficient release of bound protein under nondenaturing conditions. <ref>doi:10.1007/s00253-011-3373-5</ref> | ||
== Relevance == | == Relevance == | ||
Revision as of 10:29, 23 November 2014
Carbohydrate Binding Module (Family 3 Clostridium Thermicellum)
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References
- ↑ Guerreiro CI, Fontes CM, Gama M, Domingues L. Escherichia coli expression and purification of four antimicrobial peptides fused to a family 3 carbohydrate-binding module (CBM) from Clostridium thermocellum. Protein Expr Purif. 2008 May;59(1):161-8. doi: 10.1016/j.pep.2008.01.018. Epub, 2008 Feb 5. PMID:18328729 doi:http://dx.doi.org/10.1016/j.pep.2008.01.018
- ↑ Guerreiro CI, Fontes CM, Gama M, Domingues L. Escherichia coli expression and purification of four antimicrobial peptides fused to a family 3 carbohydrate-binding module (CBM) from Clostridium thermocellum. Protein Expr Purif. 2008 May;59(1):161-8. doi: 10.1016/j.pep.2008.01.018. Epub, 2008 Feb 5. PMID:18328729 doi:http://dx.doi.org/10.1016/j.pep.2008.01.018
- ↑ Wan W, Wang D, Gao X, Hong J. Expression of family 3 cellulose-binding module (CBM3) as an affinity tag for recombinant proteins in yeast. Appl Microbiol Biotechnol. 2011 Aug;91(3):789-98. doi: 10.1007/s00253-011-3373-5., Epub 2011 Jun 9. PMID:21656139 doi:http://dx.doi.org/10.1007/s00253-011-3373-5
