Cellulosomal scaffolding protein
From Proteopedia
(Difference between revisions)
| Line 5: | Line 5: | ||
== Function == | == Function == | ||
| - | Carbohydrate | + | Carbohydrate-binding Module |
| - | <scene name='60/609843/Beta_sheets/1'>Beta Sheets</scene> | + | The most important conformational element of most CBMs is the <scene name='60/609843/Beta_sheets/1'>Beta Sheets</scene>. t. The folds and architecture displayed by these beta-sheets have been classified into seven families with the '''β-sandwich''' being the most recurrent fold and the fold of family 3. <ref>DOI 10.1007/s00253-009-2331-y</ref> |
== Applications == | == Applications == | ||
The cellulose-binding module (CBM) is an attractive affinity tag for protein purification for several reasons: | The cellulose-binding module (CBM) is an attractive affinity tag for protein purification for several reasons: | ||
Revision as of 10:44, 23 November 2014
Carbohydrate Binding Module (Family 3 Clostridium Thermicellum)
| |||||||||||
References
- ↑ Guerreiro CI, Fontes CM, Gama M, Domingues L. Escherichia coli expression and purification of four antimicrobial peptides fused to a family 3 carbohydrate-binding module (CBM) from Clostridium thermocellum. Protein Expr Purif. 2008 May;59(1):161-8. doi: 10.1016/j.pep.2008.01.018. Epub, 2008 Feb 5. PMID:18328729 doi:http://dx.doi.org/10.1016/j.pep.2008.01.018
- ↑ Guillen D, Sanchez S, Rodriguez-Sanoja R. Carbohydrate-binding domains: multiplicity of biological roles. Appl Microbiol Biotechnol. 2010 Feb;85(5):1241-9. doi: 10.1007/s00253-009-2331-y., Epub 2009 Nov 12. PMID:19908036 doi:http://dx.doi.org/10.1007/s00253-009-2331-y
- ↑ Wan W, Wang D, Gao X, Hong J. Expression of family 3 cellulose-binding module (CBM3) as an affinity tag for recombinant proteins in yeast. Appl Microbiol Biotechnol. 2011 Aug;91(3):789-98. doi: 10.1007/s00253-011-3373-5., Epub 2011 Jun 9. PMID:21656139 doi:http://dx.doi.org/10.1007/s00253-011-3373-5
