Cellulosomal scaffolding protein

Carbohydrate-binding domain modules(CBM) are non-catalytic domain which present in glycoside hydrolases which target the associated catalytic modules to their substraes, therefore potentiating enzyme activietvarious microrganisms. CBM3 is part of CipA scafolding in Cellulosome which contain enzyme with hydrolytic activity(cellulase). ^[1]

Function

CBM3 is Type A surface-binding-planar architecture of the binding sites is thought to be complementary to the flat surfaces presented by cellulose^[2]. The binding site comprises a planar hydrophobic platform that contains three exposed aromatic amino acid The most important conformational element of most CBMs is the . The folds and architecture displayed by these beta-sheets have been classified into seven families with the β-sandwich being the most recurrent fold and the fold of family 3. ^[3]. And some .

Applications

The cellulose-binding module (CBM) is an attractive affinity tag for protein purification for several reasons: 1.Very specific binding ability to the fused protein eith CBM tag. 2.Very low nonspecific binding ability to to other protein. 3.efficient release of bound protein under nondenaturing conditions. ^[4]

Relevance

Structural highlights

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