1lua
From Proteopedia
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| - | [[Image:1lua.jpg|left|200px]] | + | [[Image:1lua.jpg|left|200px]] |
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| - | '''Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1 complexed with NADP''' | + | {{Structure |
| + | |PDB= 1lua |SIZE=350|CAPTION= <scene name='initialview01'>1lua</scene>, resolution 1.90Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NAP:NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE'>NAP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Methylenetetrahydrofolate_dehydrogenase_(NADP(+)) Methylenetetrahydrofolate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.5 1.5.1.5] | ||
| + | |GENE= mtdA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=408 Methylobacterium extorquens]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1 complexed with NADP''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LUA is a [ | + | 1LUA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methylobacterium_extorquens Methylobacterium extorquens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LUA OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of methylene-tetrahydromethanopterin dehydrogenase from methylobacterium extorquens AM1., Ermler U, Hagemeier CH, Roth A, Demmer U, Grabarse W, Warkentin E, Vorholt JA, Structure. 2002 Aug;10(8):1127-37. PMID:[http:// | + | Structure of methylene-tetrahydromethanopterin dehydrogenase from methylobacterium extorquens AM1., Ermler U, Hagemeier CH, Roth A, Demmer U, Grabarse W, Warkentin E, Vorholt JA, Structure. 2002 Aug;10(8):1127-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12176390 12176390] |
[[Category: Methylenetetrahydrofolate dehydrogenase (NADP(+))]] | [[Category: Methylenetetrahydrofolate dehydrogenase (NADP(+))]] | ||
[[Category: Methylobacterium extorquens]] | [[Category: Methylobacterium extorquens]] | ||
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[[Category: alpha/beta twisted open sheet structure]] | [[Category: alpha/beta twisted open sheet structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:35:16 2008'' |
Revision as of 10:35, 20 March 2008
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| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | mtdA (Methylobacterium extorquens) | ||||||
| Activity: | Methylenetetrahydrofolate dehydrogenase (NADP(+)), with EC number 1.5.1.5 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1 complexed with NADP
Overview
NADP-dependent methylene-H(4)MPT dehydrogenase, MtdA, from Methylobacterium extorquens AM1 catalyzes the dehydrogenation of methylene-tetrahydromethanopterin and methylene-tetrahydrofolate with NADP(+) as cosubstrate. The X-ray structure of MtdA with and without NADP bound was established at 1.9 A resolution. The enzyme is present as a homotrimer. The alpha,beta fold of the monomer is related to that of methylene-H(4)F dehydrogenases, suggesting a common evolutionary origin. The position of the active site is located within a large crevice built up by the two domains of one subunit and one domain of a second subunit. Methylene-H(4)MPT could be modeled into the cleft, and crucial active site residues such as Phe18, Lys256, His260, and Thr102 were identified. The molecular basis of the different substrate specificities and different catalytic demands of MtdA compared to methylene-H(4)F dehydrogenases are discussed.
About this Structure
1LUA is a Single protein structure of sequence from Methylobacterium extorquens. Full crystallographic information is available from OCA.
Reference
Structure of methylene-tetrahydromethanopterin dehydrogenase from methylobacterium extorquens AM1., Ermler U, Hagemeier CH, Roth A, Demmer U, Grabarse W, Warkentin E, Vorholt JA, Structure. 2002 Aug;10(8):1127-37. PMID:12176390
Page seeded by OCA on Thu Mar 20 12:35:16 2008
