3fct
From Proteopedia
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| - | [[ | + | ==MATURE METAL CHELATASE CATALYTIC ANTIBODY WITH HAPTEN== |
| + | <StructureSection load='3fct' size='340' side='right' caption='[[3fct]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3fct]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FCT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FCT FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MMP:N-METHYLMESOPORPHYRIN'>MMP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fct OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fct RCSB], [http://www.ebi.ac.uk/pdbsum/3fct PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fc/3fct_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A classic hypothesis for enzyme catalysis is the induction of strain in the substrate. This notion was first expressed by Haldane with the lock and key analogy-"the key does not fit the lock perfectly but exercises a certain strain on it" (1). This mechanism has often been invoked to explain the catalytic efficiency of enzymes but has been difficult to establish conclusively (2-7). Here we describe X-ray crystallographic and mutational studies of an antibody metal chelatase which strongly support the notion that this antibody catalyzes metal ion insertion into the porphyrin ring by inducing strain. Analysis of the germline precursor suggests that this strain mechanism arose during the process of affinity maturation in response to a conformationally distorted N-alkylmesoporphyrin. | ||
| - | + | Structural and kinetic evidence for strain in biological catalysis.,Romesberg FE, Santarsiero BD, Spiller B, Yin J, Barnes D, Schultz PG, Stevens RC Biochemistry. 1998 Oct 13;37(41):14404-9. PMID:9772166<ref>PMID:9772166</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| - | + | ==See Also== | |
| - | + | *[[Monoclonal Antibody|Monoclonal Antibody]] | |
| - | == | + | == References == |
| - | [[ | + | <references/> |
| - | + | __TOC__ | |
| - | == | + | </StructureSection> |
| - | < | + | |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Barnes, D | + | [[Category: Barnes, D]] |
| - | [[Category: Romesberg, F E | + | [[Category: Romesberg, F E]] |
| - | [[Category: Santarsiero, B D | + | [[Category: Santarsiero, B D]] |
| - | [[Category: Schultz, P G | + | [[Category: Schultz, P G]] |
| - | [[Category: Spiller, B | + | [[Category: Spiller, B]] |
| - | [[Category: Stevens, R C | + | [[Category: Stevens, R C]] |
| - | [[Category: Yin, J | + | [[Category: Yin, J]] |
[[Category: Catalytic antibody]] | [[Category: Catalytic antibody]] | ||
[[Category: Fab fragment]] | [[Category: Fab fragment]] | ||
[[Category: Immune system]] | [[Category: Immune system]] | ||
[[Category: Metal chelatase]] | [[Category: Metal chelatase]] | ||
Revision as of 08:39, 26 November 2014
MATURE METAL CHELATASE CATALYTIC ANTIBODY WITH HAPTEN
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