3g56

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[[Image:3g56.png|left|200px]]
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==Structure of the macrolide biosensor protein, MphR(A)==
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<StructureSection load='3g56' size='340' side='right' caption='[[3g56]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3g56]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G56 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3G56 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3frq|3frq]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mphR(A) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3g56 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g56 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3g56 RCSB], [http://www.ebi.ac.uk/pdbsum/3g56 PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/3g56_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The regulatory protein MphR(A) has recently seen extensive use in synthetic biological applications, such as metabolite sensing and exogenous control of gene expression. This protein negatively regulates the expression of a macrolide 2'-phosphotransferase I resistance gene (mphA) via binding to a 35-bp DNA operator upstream of the start codon and is de-repressed by the presence of erythromycin. Here, we present the refined crystal structure of the MphR(A) protein free of erythromycin and that of the MphR(A) protein with bound erythromycin at 2.00- and 1.76-A resolutions, respectively. We also studied the DNA binding properties of the protein and identified mutants of MphR(A) that are defective in gene repression and ligand binding in a cell-based reporter assay. The combination of these two structures illustrates the molecular basis of erythromycin-induced gene expression and provides a framework for additional applied uses of this protein in the isolation and engineered biosynthesis of polyketide natural products.
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{{STRUCTURE_3g56| PDB=3g56 | SCENE= }}
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Structure and function of the macrolide biosensor protein, MphR(A), with and without erythromycin.,Zheng J, Sagar V, Smolinsky A, Bourke C, LaRonde-LeBlanc N, Cropp TA J Mol Biol. 2009 Apr 17;387(5):1250-60. Epub 2009 Mar 2. PMID:19265703<ref>PMID:19265703</ref>
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===Structure of the macrolide biosensor protein, MphR(A)===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19265703}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3g56]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G56 OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019265703</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Bourke, C.]]
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[[Category: Bourke, C]]
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[[Category: Cropp, T A.]]
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[[Category: Cropp, T A]]
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[[Category: LaRonde-LeBlanc, N.]]
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[[Category: LaRonde-LeBlanc, N]]
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[[Category: Sagar, V.]]
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[[Category: Sagar, V]]
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[[Category: Smolinsky, A.]]
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[[Category: Smolinsky, A]]
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[[Category: Zheng, J.]]
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[[Category: Zheng, J]]
[[Category: Biosensor]]
[[Category: Biosensor]]
[[Category: Biosynthesis]]
[[Category: Biosynthesis]]

Revision as of 10:27, 3 December 2014

Structure of the macrolide biosensor protein, MphR(A)

3g56, resolution 2.10Å

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