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1mjg

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[[Image:1mjg.gif|left|200px]]<br /><applet load="1mjg" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1mjg.gif|left|200px]]
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caption="1mjg, resolution 2.2&Aring;" />
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'''CRYSTAL STRUCTURE OF BIFUNCTIONAL CARBON MONOXIDE DEHYDROGENASE/ACETYL-COA SYNTHASE(CODH/ACS) FROM MOORELLA THERMOACETICA (F. CLOSTRIDIUM THERMOACETICUM)'''<br />
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{{Structure
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|PDB= 1mjg |SIZE=350|CAPTION= <scene name='initialview01'>1mjg</scene>, resolution 2.2&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene> and <scene name='pdbligand=XCC:FE(4)-NI(1)-S(4) CLUSTER'>XCC</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Carbon-monoxide_dehydrogenase_(acceptor) Carbon-monoxide dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.99.2 1.2.99.2]
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|GENE=
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}}
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'''CRYSTAL STRUCTURE OF BIFUNCTIONAL CARBON MONOXIDE DEHYDROGENASE/ACETYL-COA SYNTHASE(CODH/ACS) FROM MOORELLA THERMOACETICA (F. CLOSTRIDIUM THERMOACETICUM)'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1MJG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica] with <scene name='pdbligand=CU1:'>CU1</scene>, <scene name='pdbligand=NI:'>NI</scene>, <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=SF4:'>SF4</scene> and <scene name='pdbligand=XCC:'>XCC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbon-monoxide_dehydrogenase_(acceptor) Carbon-monoxide dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.99.2 1.2.99.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJG OCA].
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1MJG is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJG OCA].
==Reference==
==Reference==
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A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase., Doukov TI, Iverson TM, Seravalli J, Ragsdale SW, Drennan CL, Science. 2002 Oct 18;298(5593):567-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12386327 12386327]
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A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase., Doukov TI, Iverson TM, Seravalli J, Ragsdale SW, Drennan CL, Science. 2002 Oct 18;298(5593):567-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12386327 12386327]
[[Category: Carbon-monoxide dehydrogenase (acceptor)]]
[[Category: Carbon-monoxide dehydrogenase (acceptor)]]
[[Category: Moorella thermoacetica]]
[[Category: Moorella thermoacetica]]
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[[Category: wood-ljundahl pathway]]
[[Category: wood-ljundahl pathway]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:44:07 2008''

Revision as of 10:44, 20 March 2008

Image:1mjg.gif


PDB ID 1mjg

Drag the structure with the mouse to rotate
, resolution 2.2Å
Ligands: , , , and
Activity: Carbon-monoxide dehydrogenase (acceptor), with EC number 1.2.99.2
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF BIFUNCTIONAL CARBON MONOXIDE DEHYDROGENASE/ACETYL-COA SYNTHASE(CODH/ACS) FROM MOORELLA THERMOACETICA (F. CLOSTRIDIUM THERMOACETICUM)


Overview

A metallocofactor containing iron, sulfur, copper, and nickel has been discovered in the enzyme carbon monoxide dehydrogenase/acetyl-CoA (coenzyme A) synthase from Moorella thermoacetica (f. Clostridium thermoaceticum). Our structure at 2.2 angstrom resolution reveals that the cofactor responsible for the assembly of acetyl-CoA contains a [Fe4S4] cubane bridged to a copper-nickel binuclear site. The presence of these three metals together in one cluster was unanticipated and suggests a newly discovered role for copper in biology. The different active sites of this bifunctional enzyme complex are connected via a channel, 138 angstroms long, that provides a conduit for carbon monoxide generated at the C-cluster on one subunit to be incorporated into acetyl-CoA at the A-cluster on the other subunit.

About this Structure

1MJG is a Protein complex structure of sequences from Moorella thermoacetica. Full crystallographic information is available from OCA.

Reference

A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase., Doukov TI, Iverson TM, Seravalli J, Ragsdale SW, Drennan CL, Science. 2002 Oct 18;298(5593):567-72. PMID:12386327

Page seeded by OCA on Thu Mar 20 12:44:07 2008

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