1mme
From Proteopedia
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| - | [[Image:1mme.jpg|left|200px]] | + | [[Image:1mme.jpg|left|200px]] |
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| - | '''THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE''' | + | {{Structure |
| + | |PDB= 1mme |SIZE=350|CAPTION= <scene name='initialview01'>1mme</scene>, resolution 3.100Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MME is a [ | + | 1MME is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. The following page contains interesting information on the relation of 1MME with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb65_1.html Self-splicing RNA]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MME OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of an all-RNA hammerhead ribozyme: a proposed mechanism for RNA catalytic cleavage., Scott WG, Finch JT, Klug A, Cell. 1995 Jun 30;81(7):991-1002. PMID:[http:// | + | The crystal structure of an all-RNA hammerhead ribozyme: a proposed mechanism for RNA catalytic cleavage., Scott WG, Finch JT, Klug A, Cell. 1995 Jun 30;81(7):991-1002. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7541315 7541315] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Self-splicing RNA]] | [[Category: Self-splicing RNA]] | ||
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[[Category: rna hammerhead ribozyme]] | [[Category: rna hammerhead ribozyme]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:45:15 2008'' |
Revision as of 10:45, 20 March 2008
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THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE
Overview
We have solved the crystal structure of an all-RNA hammerhead ribozyme having a single 2'-O-methyl cytosine incorporated at the active site to prevent cleavage. The conditions used differ from those in another recent solution in four significant ways: first, it is an all-RNA ribozyme rather than a DNA-RNA hybrid; second, the connectivity of the ribozyme backbone strands is different; third, the crystals were grown in the presence of a much lower concentration of salt; and fourth, the crystal packing scheme is very different. Nevertheless, the three-dimensional structure of the all-RNA hammerhead ribozyme is similar to the previous structure. Five potential Mg(II)-binding sites are identified, including one positioned near the ribozyme catalytic pocket. Upon this basis, as well as upon comparisons with the metal-binding sites in the structurally homologous uridine turn of tRNAPhe, we propose a mechanism for RNA catalytic cleavage.
About this Structure
1MME is a Protein complex structure of sequences from [1]. The following page contains interesting information on the relation of 1MME with [Self-splicing RNA]. Full crystallographic information is available from OCA.
Reference
The crystal structure of an all-RNA hammerhead ribozyme: a proposed mechanism for RNA catalytic cleavage., Scott WG, Finch JT, Klug A, Cell. 1995 Jun 30;81(7):991-1002. PMID:7541315
Page seeded by OCA on Thu Mar 20 12:45:15 2008
