This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3j13

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:3j13.png|left|200px]]
+
==Structural characterization of mRNA-tRNA translocation intermediates (30S ribosome of class 3 of the six classes)==
 +
<StructureSection load='3j13' size='340' side='right' caption='[[3j13]], [[Resolution|resolution]] 13.10&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3j13]] is a 23 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J13 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J13 FirstGlance]. <br>
 +
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=2MG:2N-METHYLGUANOSINE-5-MONOPHOSPHATE'>2MG</scene>, <scene name='pdbligand=4OC:4N,O2-METHYLCYTIDINE-5-MONOPHOSPHATE'>4OC</scene>, <scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MC:5-METHYLCYTIDINE-5-MONOPHOSPHATE'>5MC</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=MA6:6N-DIMETHYLADENOSINE-5-MONOPHOSHATE'>MA6</scene>, <scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=UR3:3-METHYLURIDINE-5-MONOPHOSHATE'>UR3</scene></td></tr>
 +
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2i2u|2i2u]], [[2i2v|2i2v]], [[3j0u|3j0u]], [[3j0t|3j0t]], [[3j11|3j11]], [[3j0v|3j0v]], [[3j0w|3j0w]], [[3j0x|3j0x]], [[3j0y|3j0y]], [[3j0z|3j0z]], [[3j12|3j12]], [[3j10|3j10]], [[3j14|3j14]]</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j13 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j13 RCSB], [http://www.ebi.ac.uk/pdbsum/3j13 PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Cryo-EM analysis of a wild-type Escherichia coli pretranslocational sample has revealed the presence of previously unseen intermediate substates of the bacterial ribosome during the first phase of translocation, characterized by intermediate intersubunit rotations, L1 stalk positions, and tRNA configurations. Furthermore, we describe the domain rearrangements in quantitative terms, which has allowed us to characterize the processivity and coordination of the conformational reorganization of the ribosome, along with the associated changes in tRNA ribosome-binding configuration. The results are consistent with the view of the ribosome as a molecular machine employing Brownian motion to reach a functionally productive state via a series of substates with incremental changes in conformation.
-
{{STRUCTURE_3j13| PDB=3j13 | SCENE= }}
+
Structural characterization of mRNA-tRNA translocation intermediates.,Agirrezabala X, Liao HY, Schreiner E, Fu J, Ortiz-Meoz RF, Schulten K, Green R, Frank J Proc Natl Acad Sci U S A. 2012 Mar 30. PMID:22467828<ref>PMID:22467828</ref>
-
===Structural characterization of mRNA-tRNA translocation intermediates (30S ribosome of class 3 of the six classes)===
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
{{ABSTRACT_PUBMED_22467828}}
+
-
 
+
-
==About this Structure==
+
-
[[3j13]] is a 23 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J13 OCA].
+
==See Also==
==See Also==
-
*[[Ribosomal protein S10|Ribosomal protein S10]]
+
*[[Ribosome 3D structures|Ribosome 3D structures]]
-
*[[Ribosomal protein S11|Ribosomal protein S11]]
+
== References ==
-
*[[Ribosomal protein S12|Ribosomal protein S12]]
+
<references/>
-
*[[Ribosomal protein S13|Ribosomal protein S13]]
+
__TOC__
-
*[[Ribosomal protein S14|Ribosomal protein S14]]
+
</StructureSection>
-
*[[Ribosomal protein S15|Ribosomal protein S15]]
+
-
*[[Ribosomal protein S16|Ribosomal protein S16]]
+
-
*[[Ribosomal protein S17|Ribosomal protein S17]]
+
-
*[[Ribosomal protein S18|Ribosomal protein S18]]
+
-
*[[Ribosomal protein S19|Ribosomal protein S19]]
+
-
*[[Ribosomal protein S2|Ribosomal protein S2]]
+
-
*[[Ribosomal protein S20|Ribosomal protein S20]]
+
-
*[[Ribosomal protein S21|Ribosomal protein S21]]
+
-
*[[Ribosomal protein S3|Ribosomal protein S3]]
+
-
*[[Ribosomal protein S4|Ribosomal protein S4]]
+
-
*[[Ribosomal protein S5|Ribosomal protein S5]]
+
-
*[[Ribosomal protein S6|Ribosomal protein S6]]
+
-
*[[Ribosomal protein S7|Ribosomal protein S7]]
+
-
*[[Ribosomal protein S8|Ribosomal protein S8]]
+
-
*[[Ribosomal protein S9|Ribosomal protein S9]]
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:022467828</ref><references group="xtra"/>
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Agirrezabala, X.]]
+
[[Category: Agirrezabala, X]]
-
[[Category: Frank, J.]]
+
[[Category: Frank, J]]
-
[[Category: Fu, J.]]
+
[[Category: Fu, J]]
-
[[Category: Green, R.]]
+
[[Category: Green, R]]
-
[[Category: Liao, H.]]
+
[[Category: Liao, H]]
-
[[Category: Ortiz-Meoz, R F.]]
+
[[Category: Ortiz-Meoz, R F]]
-
[[Category: Schreiner, E.]]
+
[[Category: Schreiner, E]]
-
[[Category: Schulten, K.]]
+
[[Category: Schulten, K]]
[[Category: Cryoelectron microscopy]]
[[Category: Cryoelectron microscopy]]
[[Category: Molecular dynamics flexible fitting]]
[[Category: Molecular dynamics flexible fitting]]
[[Category: Ratchet-like rotation]]
[[Category: Ratchet-like rotation]]
[[Category: Ribosome]]
[[Category: Ribosome]]

Revision as of 08:57, 8 December 2014

Structural characterization of mRNA-tRNA translocation intermediates (30S ribosome of class 3 of the six classes)

3j13, resolution 13.10Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3j13"
Personal tools