1mwz
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1mwz.jpg|left|200px]] | + | [[Image:1mwz.jpg|left|200px]] |
| - | + | ||
| - | '''Solution structure of the N-terminal domain of ZntA in the Zn(II)-form''' | + | {{Structure |
| + | |PDB= 1mwz |SIZE=350|CAPTION= <scene name='initialview01'>1mwz</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= znta ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Solution structure of the N-terminal domain of ZntA in the Zn(II)-form''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1MWZ is a [ | + | 1MWZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWZ OCA]. |
==Reference== | ==Reference== | ||
| - | A new zinc-protein coordination site in intracellular metal trafficking: solution structure of the Apo and Zn(II) forms of ZntA(46-118)., Banci L, Bertini I, Ciofi-Baffoni S, Finney LA, Outten CE, O'Halloran TV, J Mol Biol. 2002 Nov 8;323(5):883-97. PMID:[http:// | + | A new zinc-protein coordination site in intracellular metal trafficking: solution structure of the Apo and Zn(II) forms of ZntA(46-118)., Banci L, Bertini I, Ciofi-Baffoni S, Finney LA, Outten CE, O'Halloran TV, J Mol Biol. 2002 Nov 8;323(5):883-97. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12417201 12417201] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 23: | Line 32: | ||
[[Category: open-faced beta-sandwich fold]] | [[Category: open-faced beta-sandwich fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:49:15 2008'' |
Revision as of 10:49, 20 March 2008
| |||||||
| Ligands: | |||||||
| Gene: | znta (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of the N-terminal domain of ZntA in the Zn(II)-form
Overview
Zinc, a metal ion that functions in a wide variety of catalytic and structural sites in metalloproteins, is shown here to adopt a novel coordination environment in the Escherichia coli transport protein ZntA. The ZntA protein is a P-type ATPase that pumps zinc out of the cytoplasm and into the periplasm. It is physiologically selective for Zn(II) and functions with metalloregulatory proteins in the cell to keep the zinc quota within strict limits. Yet, the N-terminal cytoplasmic domain contains a region that is highly homologous to the yeast Cu(I) metallochaperone Atx1. To investigate how the structure of this region may influence its function, this fragment, containing residues 46-118, has been cloned out of the gene and overexpressed. We report here the solution structure of this fragment as determined by NMR. Both the apo and Zn(II)-ZntA(46-118) structures have been determined. It contains a previously unknown protein coordination site for zinc that includes two cysteine residues, Cys59 and Cys62, and a carboxylate residue, Asp58. The solvent accessibility of this site is also remarkably high, a feature that increasingly appears to be a characteristic of domains of heavy metal ion transport proteins. The participation of Asp58 in this ZntA metal ion binding site may play an important role in modulating the relative affinities and metal exchange rates for Zn(II)/Pb(II)/Cd(II) as compared with other P-type ATPases, which are selective for Cu(I) or Ag(I).
About this Structure
1MWZ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
A new zinc-protein coordination site in intracellular metal trafficking: solution structure of the Apo and Zn(II) forms of ZntA(46-118)., Banci L, Bertini I, Ciofi-Baffoni S, Finney LA, Outten CE, O'Halloran TV, J Mol Biol. 2002 Nov 8;323(5):883-97. PMID:12417201
Page seeded by OCA on Thu Mar 20 12:49:15 2008
