1n4l
From Proteopedia
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| - | [[Image:1n4l.gif|left|200px]] | + | [[Image:1n4l.gif|left|200px]] |
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| - | '''A DNA analogue of the polypurine tract of HIV-1''' | + | {{Structure |
| + | |PDB= 1n4l |SIZE=350|CAPTION= <scene name='initialview01'>1n4l</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''A DNA analogue of the polypurine tract of HIV-1''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1N4L is a [ | + | 1N4L is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4L OCA]. |
==Reference== | ==Reference== | ||
| - | Staying straight with A-tracts: a DNA analog of the HIV-1 polypurine tract., Cote ML, Pflomm M, Georgiadis MM, J Mol Biol. 2003 Jun 27;330(1):57-74. PMID:[http:// | + | Staying straight with A-tracts: a DNA analog of the HIV-1 polypurine tract., Cote ML, Pflomm M, Georgiadis MM, J Mol Biol. 2003 Jun 27;330(1):57-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12818202 12818202] |
[[Category: Moloney murine leukemia virus]] | [[Category: Moloney murine leukemia virus]] | ||
[[Category: RNA-directed DNA polymerase]] | [[Category: RNA-directed DNA polymerase]] | ||
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[[Category: mmlv rt; polypurine tract; hiv-1; asymmetric dna; protein-dna complex]] | [[Category: mmlv rt; polypurine tract; hiv-1; asymmetric dna; protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:52:04 2008'' |
Revision as of 10:52, 20 March 2008
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| , resolution 2.00Å | |||||||
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| Activity: | RNA-directed DNA polymerase, with EC number 2.7.7.49 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
A DNA analogue of the polypurine tract of HIV-1
Overview
The polypurine tract (PPT) from the HIV-1 genome is resistant to digestion by reverse transcriptase following (-)-strand synthesis and is used to prime (+)-strand synthesis during retroviral replication. We have determined the crystal structure of the asymmetric DNA/DNA analog16-mer duplex (CTTTTTAAAAGAAAAG/CTTTTCTTTTAAAAAG) comprising most of the "visible" portion of the RNA:DNA hybrid from the polypurine tract of HIV-1, which was previously reported in a complex with HIV-1 reverse transcriptase. Our 16-mer completely encompasses a 10-mer DNA duplex analog of the HIV-1 PPT. We report here a detailed analysis of our B' form 16-mer DNA structure, including three full pure A-tracts, as well as a comparative structural analysis with polypurine tract and other A-tract-containing nucleic acid structures. Our analysis reveals that the polypurine tract structures share structural features despite being different nucleic acid forms (i.e. DNA:DNA versus RNA:DNA). In addition, the previously reported A-tract-containing DNA molecules bound to topoisomerase I are remarkably similar to our polypurine tract 16-mer structure. On the basis of our analysis, we suggest that the specific topology of long pure A-tracts is remarkably comparable across a wide array of biological environments.
About this Structure
1N4L is a Single protein structure of sequence from Moloney murine leukemia virus. Full crystallographic information is available from OCA.
Reference
Staying straight with A-tracts: a DNA analog of the HIV-1 polypurine tract., Cote ML, Pflomm M, Georgiadis MM, J Mol Biol. 2003 Jun 27;330(1):57-74. PMID:12818202
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