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1nhl

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[[Image:1nhl.jpg|left|200px]]<br /><applet load="1nhl" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1nhl.jpg|left|200px]]
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caption="1nhl, resolution 2.3&Aring;" />
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'''SNAP-23N Structure'''<br />
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{{Structure
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|PDB= 1nhl |SIZE=350|CAPTION= <scene name='initialview01'>1nhl</scene>, resolution 2.3&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE= SNAP23 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''SNAP-23N Structure'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1NHL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NHL OCA].
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1NHL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NHL OCA].
==Reference==
==Reference==
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Homotetrameric structure of the SNAP-23 N-terminal coiled-coil domain., Freedman SJ, Song HK, Xu Y, Sun ZY, Eck MJ, J Biol Chem. 2003 Apr 11;278(15):13462-7. Epub 2003 Jan 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12556468 12556468]
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Homotetrameric structure of the SNAP-23 N-terminal coiled-coil domain., Freedman SJ, Song HK, Xu Y, Sun ZY, Eck MJ, J Biol Chem. 2003 Apr 11;278(15):13462-7. Epub 2003 Jan 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12556468 12556468]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: snare]]
[[Category: snare]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:06:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:57:00 2008''

Revision as of 10:57, 20 March 2008

Image:1nhl.jpg


PDB ID 1nhl

Drag the structure with the mouse to rotate
, resolution 2.3Å
Gene: SNAP23 (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



SNAP-23N Structure


Overview

SNARE proteins mediate intracellular membrane fusion by forming a coiled-coil complex to merge opposing membranes. A "fusion-active" neuronal SNARE complex is a parallel four-helix bundle containing two coiled-coil domains from SNAP-25 and one coiled-coil domain each from syntaxin-1a and VAMP-2. "Prefusion" assembly intermediate complexes can also form from these SNAREs. We studied the N-terminal coiled-coil domain of SNAP-23 (SNAP-23N), a non-neuronal homologue of SNAP-25, and its interaction with other coiled-coil domains. SNAP-23N can assemble spontaneously with the coiled-coil domains from SNAP-23C, syntaxin-4, and VAMP-3 to form a heterotetrameric complex. Unexpectedly, pure SNAP-23N crystallizes as a coiled-coil homotetrameric complex. The four helices have a parallel orientation and are symmetrical about the long axis. The complex is stabilized through the interaction of conserved hydrophobic residues comprising the a and d positions of the coiled-coil heptad repeats. In addition, a central, highly conserved glutamine residue (Gln-48) is buried within the interface by hydrogen bonding between glutamine side chains derived from adjacent subunits and to solvent molecules. A comparison of the SNAP-23N structure to other SNARE complex structures reveals how a simple coiled-coil motif can form diverse SNARE complexes.

About this Structure

1NHL is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Homotetrameric structure of the SNAP-23 N-terminal coiled-coil domain., Freedman SJ, Song HK, Xu Y, Sun ZY, Eck MJ, J Biol Chem. 2003 Apr 11;278(15):13462-7. Epub 2003 Jan 29. PMID:12556468

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