1e4e
From Proteopedia
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1E4E is a | + | 1E4E is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium] with MG, SO4, ADP, PHY and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Sites: AC1, AC2, AC3 and AC4. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E4E OCA]. |
==Reference== | ==Reference== | ||
| Line 27: | Line 27: | ||
[[Category: plasmid]] | [[Category: plasmid]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 12:24:06 2007'' |
Revision as of 10:18, 5 November 2007
|
D-ALANYL-D-LACATE LIGASE
Overview
d-alanine-d-lactate ligase from Enterococcus faecium BM4147 is directly, responsible for the biosynthesis of alternate cell-wall precursors in, bacteria, which are resistant to the glycopeptide antibiotic vancomycin., The crystal structure has been determined with data extending to 2.5-A, resolution. This structure shows that the active site has unexpected, interactions and is distinct from previous models for d-alanyl-d-lactate, ligase mechanistic studies. It appears that the preference of the enzyme, for lactate as a ligand over d-alanine could be mediated by electrostatic, effects and/or a hydrogen-bonding network, which principally involve, His-244. The structure of d-alanyl-d-lactate ligase provides a revised, interpretation of the molecular events that lead to vancomycin resistance.
About this Structure
1E4E is a Protein complex structure of sequences from Enterococcus faecium with MG, SO4, ADP, PHY and GOL as ligands. Structure known Active Sites: AC1, AC2, AC3 and AC4. Full crystallographic information is available from OCA.
Reference
The molecular basis of vancomycin resistance in clinically relevant Enterococci: crystal structure of D-alanyl-D-lactate ligase (VanA)., Roper DI, Huyton T, Vagin A, Dodson G, Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8921-5. PMID:10908650
Page seeded by OCA on Mon Nov 5 12:24:06 2007
Categories: Enterococcus faecium | Protein complex | Roper, D.I. | ADP | GOL | MG | PHY | SO4 | Antibiotic resistance | Cell wall | Ligase | Membrane | Peptidoglycan synthesis | Plasmid
