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3ub0

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[[Image:3ub0.png|left|200px]]
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==Crystal structure of the nonstructural protein 7 and 8 complex of Feline Coronavirus==
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<StructureSection load='3ub0' size='340' side='right' caption='[[3ub0]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3ub0]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Feline_infectious_peritonitis_virus_(strain_79-1146) Feline infectious peritonitis virus (strain 79-1146)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UB0 FirstGlance]. <br>
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rep, 1a-1b ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=33734 Feline infectious peritonitis virus (strain 79-1146)])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ub0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ub0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ub0 RCSB], [http://www.ebi.ac.uk/pdbsum/3ub0 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Non-structural proteins 7 and 8 of SARS coronavirus have previously been shown by X-ray crystallography to form an 8:8 hexadecamer. In addition, it has been demonstrated that N-terminally His(6)-tagged SARS-CoV Nsp8 is a primase able to synthesize RNA oligonucleotides with a length of up to 6 nucleotides. Here we present the 2.6-A crystal structure of the Feline Coronavirus (FCoV) Nsp7:Nsp8 complex, which is a 2:1 heterotrimer containing two copies of the alpha-helical Nsp7 with conformational differences between them, and one copy of Nsp8 that consists of an alpha/beta domain and a long-alpha-helix domain. The same stoichiometry is found for the Nsp7:Nsp8 complex in solution, as demonstrated by chemical cross-linking, size-exclusion chromatography, and small-angle X-ray scattering. Furthermore, we show that FCoV Nsp8, like its SARS-CoV counterpart, is able to synthesize short oligoribomucleotides of up to 6 nucleotides in length when carrying an N-terminal hexahistidine tag. Remarkably, the same protein harbouring the sequence GPLG instead of the His(6)-tag at its N-terminus exhibits a substantially increased, primer-independent RNA polymerase activity. Upon addition of Nsp7, the RNA polymerase activity is further enhanced so that RNA up to template-length (67 nucleotides) can be synthesized. Further, we show that the unprocessed intermediate polyprotein Nsp7-10 of Human Coronavirus (HCoV) 229E is also capable of synthesizing oligoribonucleotides up to a chain-length of six. These results indicate that in case of FCoV as well as HCoV 229E, the formation of a hexadecameric Nsp7:Nsp8 complex is not necessary for RNA polymerase activity. Further, the FCoV Nsp7:Nsp8 complex functions as a non-canonical RNA polymerase capable of synthesizing RNA of up to template-length.
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{{STRUCTURE_3ub0| PDB=3ub0 | SCENE= }}
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Non-structural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer that Exhibits Primer-independent RNA Polymerase Activity.,Xiao Y, Ma Q, Restle T, Shang W, Svergun DI, Ponnusamy R, Sczakiel G, Hilgenfeld R J Virol. 2012 Feb 8. PMID:22318142<ref>PMID:22318142</ref>
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===Crystal structure of the nonstructural protein 7 and 8 complex of Feline Coronavirus===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_22318142}}
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==About this Structure==
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[[3ub0]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Feline_infectious_peritonitis_virus_(strain_79-1146) Feline infectious peritonitis virus (strain 79-1146)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UB0 OCA].
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==See Also==
==See Also==
*[[Nonstructural protein|Nonstructural protein]]
*[[Nonstructural protein|Nonstructural protein]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:022318142</ref><references group="xtra"/>
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__TOC__
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[[Category: Hilgenfeld, R.]]
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</StructureSection>
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[[Category: Ma, Q.]]
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[[Category: Hilgenfeld, R]]
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[[Category: Xiao, Y.]]
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[[Category: Ma, Q]]
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[[Category: Xiao, Y]]
[[Category: Feline coronavirus]]
[[Category: Feline coronavirus]]
[[Category: Nonstructural protein]]
[[Category: Nonstructural protein]]
[[Category: Primer-independent rna polymerase]]
[[Category: Primer-independent rna polymerase]]
[[Category: Replication]]
[[Category: Replication]]

Revision as of 17:31, 9 December 2014

Crystal structure of the nonstructural protein 7 and 8 complex of Feline Coronavirus

3ub0, resolution 2.60Å

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