|
|
| (8 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:1s1h.gif|left|200px]]
| + | #REDIRECT [[4v4b]] This PDB entry is obsolete and replaced by 4v4b |
| - | | + | |
| - | {{Structure
| + | |
| - | |PDB= 1s1h |SIZE=350|CAPTION= <scene name='initialview01'>1s1h</scene>
| + | |
| - | |SITE=
| + | |
| - | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
| + | |
| - | |ACTIVITY=
| + | |
| - | |GENE=
| + | |
| - | |DOMAIN=
| + | |
| - | |RELATEDENTRY=[[1k5x|1K5X]], [[1k5y|1K5Y]], [[1mms|1MMS]], [[1mzp|1MZP]], [[1ffk|1FFK]], [[1fjf|1FJF]], [[1fka|1FKA]], [[1gix|1GIX]], [[1giy|1GIY]], [[1nou|1NOU]], [[1s1i|1S1I]]
| + | |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s1h OCA], [http://www.ebi.ac.uk/pdbsum/1s1h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s1h RCSB]</span>
| + | |
| - | }}
| + | |
| - | | + | |
| - | '''Structure of the ribosomal 80S-eEF2-sordarin complex from yeast obtained by docking atomic models for RNA and protein components into a 11.7 A cryo-EM map. This file, 1S1H, Contains 40S subunit. The 60S Ribosomal Subunit Is In File 1S1I.'''
| + | |
| - | | + | |
| - | | + | |
| - | ==Overview==
| + | |
| - | An 11.7-A-resolution cryo-EM map of the yeast 80S.eEF2 complex in the presence of the antibiotic sordarin was interpreted in molecular terms, revealing large conformational changes within eEF2 and the 80S ribosome, including a rearrangement of the functionally important ribosomal intersubunit bridges. Sordarin positions domain III of eEF2 so that it can interact with the sarcin-ricin loop of 25S rRNA and protein rpS23 (S12p). This particular conformation explains the inhibitory action of sordarin and suggests that eEF2 is stalled on the 80S ribosome in a conformation that has similarities with the GTPase activation state. A ratchet-like subunit rearrangement (RSR) occurs in the 80S.eEF2.sordarin complex that, in contrast to Escherichia coli 70S ribosomes, is also present in vacant 80S ribosomes. A model is suggested, according to which the RSR is part of a mechanism for moving the tRNAs during the translocation reaction.
| + | |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1S1H is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S1H OCA].
| + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation., Spahn CM, Gomez-Lorenzo MG, Grassucci RA, Jorgensen R, Andersen GR, Beckmann R, Penczek PA, Ballesta JP, Frank J, EMBO J. 2004 Mar 10;23(5):1008-19. Epub 2004 Feb 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14976550 14976550]
| + | |
| - | [[Category: Protein complex]]
| + | |
| - | [[Category: Saccharomyces cerevisiae]]
| + | |
| - | [[Category: Andersen, G R.]]
| + | |
| - | [[Category: Ballesta, J P.G.]]
| + | |
| - | [[Category: Beckmann, R.]]
| + | |
| - | [[Category: Frank, J.]]
| + | |
| - | [[Category: Gomez-Lorenzo, M G.]]
| + | |
| - | [[Category: Grassucci, R A.]]
| + | |
| - | [[Category: Jorgensen, R.]]
| + | |
| - | [[Category: Penczek, P A.]]
| + | |
| - | [[Category: Spahn, C M.]]
| + | |
| - | [[Category: 40s ribosomal subunit]]
| + | |
| - | [[Category: 80s ribosome]]
| + | |
| - | [[Category: cryo-em]]
| + | |
| - | [[Category: eef2]]
| + | |
| - | [[Category: sordarin]]
| + | |
| - | [[Category: trna translocation]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:36:02 2008''
| + | |
