|
|
| Line 1: |
Line 1: |
| - | ==Structure of the Drosophila apoptosome==
| + | #REDIRECT [[4v4l]] This PDB entry is obsolete and replaced by 4v4l |
| - | <StructureSection load='1vt4' size='340' side='right' caption='[[1vt4]], [[Resolution|resolution]] 6.90Å' scene=''>
| + | |
| - | == Structural highlights ==
| + | |
| - | <table><tr><td colspan='2'>[[1vt4]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VT4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VT4 FirstGlance]. <br>
| + | |
| - | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTP:2-DEOXYADENOSINE+5-TRIPHOSPHATE'>DTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
| + | |
| - | <tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
| + | |
| - | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3iz8|3iz8]]</td></tr>
| + | |
| - | <tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ark, Hac1, dapaf-1L, CG6829, Dmel_CG6829 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
| + | |
| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vt4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vt4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vt4 RCSB], [http://www.ebi.ac.uk/pdbsum/1vt4 PDBsum]</span></td></tr>
| + | |
| - | <table>
| + | |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The Drosophila Apaf-1 related killer forms an apoptosome in the intrinsic cell death pathway. In this study we show that Dark forms a single ring when initiator procaspases are bound. This Dark-Dronc complex cleaves DrICE efficiently; hence, a single ring represents the Drosophila apoptosome. We then determined the 3D structure of a double ring at approximately 6.9 A resolution and created a model of the apoptosome. Subunit interactions in the Dark complex are similar to those in Apaf-1 and CED-4 apoptosomes, but there are significant differences. In particular, Dark has "lost" a loop in the nucleotide-binding pocket, which opens a path for possible dATP exchange in the apoptosome. In addition, caspase recruitment domains (CARDs) form a crown on the central hub of the Dark apoptosome. This CARD geometry suggests that conformational changes will be required to form active Dark-Dronc complexes. When taken together, these data provide insights into apoptosome structure, function, and evolution.
| + | |
| - | | + | |
| - | Structure of the Drosophila apoptosome at 6.9 a resolution.,Yuan S, Yu X, Topf M, Dorstyn L, Kumar S, Ludtke SJ, Akey CW Structure. 2011 Jan 12;19(1):128-40. PMID:21220123<ref>PMID:21220123</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | | + | |
| - | ==See Also==
| + | |
| - | *[[Cell death protein|Cell death protein]]
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| - | __TOC__
| + | |
| - | </StructureSection>
| + | |
| - | [[Category: Drome]]
| + | |
| - | [[Category: Akey, C W.]]
| + | |
| - | [[Category: Ludtke, S J.]]
| + | |
| - | [[Category: Topf, M.]]
| + | |
| - | [[Category: Yuan, S.]]
| + | |
| - | [[Category: Apoptosis]]
| + | |
| - | [[Category: Drosophila apoptosome]]
| + | |
| - | [[Category: Programmed cell death]]
| + | |
