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| - | [[Image:2uva.gif|left|200px]]<br /><applet load="2uva" size="350" color="white" frame="true" align="right" spinBox="true" | + | #REDIRECT [[4v58]] This PDB entry is obsolete and replaced by 4v58 |
| - | caption="2uva, resolution 3.1Å" />
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| - | '''CRYSTAL STRUCTURE OF FATTY ACID SYNTHASE FROM THERMOMYCES LANUGINOSUS AT 3.1 ANGSTROM RESOLUTION. THIS FILE CONTAINS THE BETA SUBUNITS OF THE FATTY ACID SYNTHASE. THE ENTIRE CRYSTAL STRUCTURE CONSISTS OF ONE HETERODODECAMERIC FATTY ACID SYNTHASE AND IS DESCRIBED IN REMARK 400'''<br />
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| - | ==Overview==
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| - | We report crystal structures of the 2.6-megadalton alpha6beta6 heterododecameric fatty acid synthase from Thermomyces lanuginosus at 3.1 angstrom resolution. The alpha and beta polypeptide chains form the six catalytic domains required for fatty acid synthesis and numerous expansion segments responsible for extensive intersubunit connections. Detailed views of all active sites provide insights into substrate specificities and catalytic mechanisms and reveal their unique characteristics, which are due to the integration into the multienzyme. The mode of acyl carrier protein attachment in the reaction chamber, together with the spatial distribution of active sites, suggests that iterative substrate shuttling is achieved by a relatively restricted circular motion of the carrier domain in the multifunctional enzyme.
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| - | ==About this Structure==
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| - | 2UVA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermomyces_lanuginosus Thermomyces lanuginosus] with <scene name='pdbligand=FMN:'>FMN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Fmn+Binding+Site+For+Chain+G'>AC1</scene>, <scene name='pdbsite=AC2:Fmn+Binding+Site+For+Chain+H'>AC2</scene>, <scene name='pdbsite=AC3:Fmn+Binding+Site+For+Chain+I'>AC3</scene>, <scene name='pdbsite=AC4:Fmn+Binding+Site+For+Chain+J'>AC4</scene>, <scene name='pdbsite=AC5:Fmn+Binding+Site+For+Chain+K'>AC5</scene> and <scene name='pdbsite=AC6:Fmn+Binding+Site+For+Chain+L'>AC6</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVA OCA].
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| - | ==Reference==
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| - | Structure of fungal fatty acid synthase and implications for iterative substrate shuttling., Jenni S, Leibundgut M, Boehringer D, Frick C, Mikolasek B, Ban N, Science. 2007 Apr 13;316(5822):254-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17431175 17431175]
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| - | [[Category: Single protein]]
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| - | [[Category: Thermomyces lanuginosus]]
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| - | [[Category: Ban, N.]]
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| - | [[Category: Boehringer, D.]]
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| - | [[Category: Frick, C.]]
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| - | [[Category: Jenni, S.]]
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| - | [[Category: Leibundgut, M.]]
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| - | [[Category: Mikolasek, B.]]
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| - | [[Category: FMN]]
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| - | [[Category: acetyl transferase]]
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| - | [[Category: acyl carrier protein]]
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| - | [[Category: dehydratase]]
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| - | [[Category: enoyl reductase]]
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| - | [[Category: fatty acid synthase]]
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| - | [[Category: fatty acid synthesis]]
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| - | [[Category: fungal]]
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| - | [[Category: ketoacyl reductase]]
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| - | [[Category: ketoacyl synthase]]
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| - | [[Category: malonyl/palmitoyl transferase]]
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| - | [[Category: multifunctional enzyme]]
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| - | [[Category: substrate shuttling]]
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| - | [[Category: transferase]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:50:52 2008''
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