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| - | ==Structure of the Thermus thermophilus ribosome complexed with erythromycin. This file contains the 30S subunit of one 70S ribosome. The entire crystal structure contains two 70S ribosomes.==
| + | #REDIRECT [[4v7x]] This PDB entry is obsolete and replaced by 4v7x |
| - | <StructureSection load='3ohc' size='340' side='right' caption='[[3ohc]], [[Resolution|resolution]] 3.00Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[3ohc]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OHC FirstGlance]. <br>
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| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3oge|3oge]], [[3ogy|3ogy]], [[3oh5|3oh5]], [[3oh7|3oh7]], [[3ohd|3ohd]], [[3ohj|3ohj]], [[3ohk|3ohk]], [[3ohy|3ohy]], [[3ohz|3ohz]], [[3oi0|3oi0]], [[3oi1|3oi1]], [[3oi2|3oi2]], [[3oi3|3oi3]], [[3oi4|3oi4]], [[3oi5|3oi5]], [[3os1|3os1]], [[3os2|3os2]]</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ohc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ohc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ohc RCSB], [http://www.ebi.ac.uk/pdbsum/3ohc PDBsum]</span></td></tr>
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| - | </table>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | The increasing prevalence of antibiotic-resistant pathogens reinforces the need for structures of antibiotic-ribosome complexes that are accurate enough to enable the rational design of novel ribosome-targeting therapeutics. Structures of many antibiotics in complex with both archaeal and eubacterial ribosomes have been determined, yet discrepancies between several of these models have raised the question of whether these differences arise from species-specific variations or from experimental problems. Our structure of chloramphenicol in complex with the 70S ribosome from Thermus thermophilus suggests a model for chloramphenicol bound to the large subunit of the bacterial ribosome that is radically different from the prevailing model. Further, our structures of the macrolide antibiotics erythromycin and azithromycin in complex with a bacterial ribosome are indistinguishable from those determined of complexes with the 50S subunit of Haloarcula marismortui, but differ significantly from the models that have been published for 50S subunit complexes of the eubacterium Deinococcus radiodurans. Our structure of the antibiotic telithromycin bound to the T. thermophilus ribosome reveals a lactone ring with a conformation similar to that observed in the H. marismortui and D. radiodurans complexes. However, the alkyl-aryl moiety is oriented differently in all three organisms, and the contacts observed with the T. thermophilus ribosome are consistent with biochemical studies performed on the Escherichia coli ribosome. Thus, our results support a mode of macrolide binding that is largely conserved across species, suggesting that the quality and interpretation of electron density, rather than species specificity, may be responsible for many of the discrepancies between the models.
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| - | Revisiting the structures of several antibiotics bound to the bacterial ribosome.,Bulkley D, Innis CA, Blaha G, Steitz TA Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17158-63. Epub 2010 Sep 27. PMID:20876130<ref>PMID:20876130</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | ==See Also==
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| - | *[[Ribosomal protein THX|Ribosomal protein THX]]
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| - | *[[Ribosome 3D structures|Ribosome 3D structures]]
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Thermus thermophilus]]
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| - | [[Category: Blaha, G]]
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| - | [[Category: Bulkley, D P]]
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| - | [[Category: Innis, C A]]
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| - | [[Category: Steitz, T A]]
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| - | [[Category: Erythromycin]]
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| - | [[Category: Protein synthesis]]
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| - | [[Category: Ribosome]]
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| - | [[Category: Ribosome-antibiotic complex]]
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