|
|
| Line 1: |
Line 1: |
| - | ==Crystal structure of HPF bound to the 70S ribosome. This PDB entry contains coordinates for the 30S subunit with bound HPF of the 1st ribosome in the ASU==
| + | #REDIRECT [[4v8h]] This PDB entry is obsolete and replaced by 4v8h |
| - | <StructureSection load='3v26' size='340' side='right' caption='[[3v26]], [[Resolution|resolution]] 3.10Å' scene=''>
| + | |
| - | == Structural highlights ==
| + | |
| - | <table><tr><td colspan='2'>[[3v26]] is a 22 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V26 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V26 FirstGlance]. <br>
| + | |
| - | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
| + | |
| - | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3v27|3v27]], [[3v28|3v28]], [[3v29|3v29]]</td></tr>
| + | |
| - | <tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b3203, hpf, JW3170, yhbH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
| + | |
| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v26 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v26 RCSB], [http://www.ebi.ac.uk/pdbsum/3v26 PDBsum]</span></td></tr>
| + | |
| - | <table>
| + | |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Eubacteria inactivate their ribosomes as 100S dimers or 70S monomers upon entry into stationary phase. In Escherichia coli, 100S dimer formation is mediated by ribosome modulation factor (RMF) and hibernation promoting factor (HPF), or alternatively, the YfiA protein inactivates ribosomes as 70S monomers. Here, we present high-resolution crystal structures of the Thermus thermophilus 70S ribosome in complex with each of these stationary-phase factors. The binding site of RMF overlaps with that of the messenger RNA (mRNA) Shine-Dalgarno sequence, which prevents the interaction between the mRNA and the 16S ribosomal RNA. The nearly identical binding sites of HPF and YfiA overlap with those of the mRNA, transfer RNA, and initiation factors, which prevents translation initiation. The binding of RMF and HPF, but not YfiA, to the ribosome induces a conformational change of the 30S head domain that promotes 100S dimer formation.
| + | |
| - | | + | |
| - | How hibernation factors RMF, HPF, and YfiA turn off protein synthesis.,Polikanov YS, Blaha GM, Steitz TA Science. 2012 May 18;336(6083):915-8. PMID:22605777<ref>PMID:22605777</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | | + | |
| - | ==See Also==
| + | |
| - | *[[Ribosomal protein THX|Ribosomal protein THX]]
| + | |
| - | *[[Ribosome 3D structures|Ribosome 3D structures]]
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| - | __TOC__
| + | |
| - | </StructureSection>
| + | |
| - | [[Category: Escherichia coli]]
| + | |
| - | [[Category: Thermus thermophilus]]
| + | |
| - | [[Category: Blaha, G M.]]
| + | |
| - | [[Category: Polikanov, Y S.]]
| + | |
| - | [[Category: Steitz, T A.]]
| + | |
| - | [[Category: Protein e]]
| + | |
| - | [[Category: Ribosome]]
| + | |
| - | [[Category: Ribosome hibernation]]
| + | |
| - | [[Category: Ribosome hibernation factor]]
| + | |
| - | [[Category: Stationary phase]]
| + | |
| - | [[Category: Stress response]]
| + | |
| - | [[Category: Yhbh]]
| + | |
