1op3

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[[Image:1op3.jpg|left|200px]]<br /><applet load="1op3" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1op3.jpg|left|200px]]
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caption="1op3, resolution 1.75&Aring;" />
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'''Crystal Structure of Fab 2G12 bound to Man1->2Man'''<br />
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{{Structure
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|PDB= 1op3 |SIZE=350|CAPTION= <scene name='initialview01'>1op3</scene>, resolution 1.75&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene> and <scene name='pdbligand=BEZ:BENZOIC ACID'>BEZ</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Crystal Structure of Fab 2G12 bound to Man1->2Man'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1OP3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MAN:'>MAN</scene> and <scene name='pdbligand=BEZ:'>BEZ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OP3 OCA].
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1OP3 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OP3 OCA].
==Reference==
==Reference==
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Antibody domain exchange is an immunological solution to carbohydrate cluster recognition., Calarese DA, Scanlan CN, Zwick MB, Deechongkit S, Mimura Y, Kunert R, Zhu P, Wormald MR, Stanfield RL, Roux KH, Kelly JW, Rudd PM, Dwek RA, Katinger H, Burton DR, Wilson IA, Science. 2003 Jun 27;300(5628):2065-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12829775 12829775]
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Antibody domain exchange is an immunological solution to carbohydrate cluster recognition., Calarese DA, Scanlan CN, Zwick MB, Deechongkit S, Mimura Y, Kunert R, Zhu P, Wormald MR, Stanfield RL, Roux KH, Kelly JW, Rudd PM, Dwek RA, Katinger H, Burton DR, Wilson IA, Science. 2003 Jun 27;300(5628):2065-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12829775 12829775]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: domain-swapped fab 2g12; anti-carbohydrate antibody]]
[[Category: domain-swapped fab 2g12; anti-carbohydrate antibody]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:20:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:13:44 2008''

Revision as of 11:13, 20 March 2008

Image:1op3.jpg


PDB ID 1op3

Drag the structure with the mouse to rotate
, resolution 1.75Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of Fab 2G12 bound to Man1->2Man


Overview

Human antibody 2G12 neutralizes a broad range of human immunodeficiency virus type 1 (HIV-1) isolates by binding an unusually dense cluster of carbohydrate moieties on the "silent" face of the gp120 envelope glycoprotein. Crystal structures of Fab 2G12 and its complexes with the disaccharide Manalpha1-2Man and with the oligosaccharide Man9GlcNAc2 revealed that two Fabs assemble into an interlocked VH domain-swapped dimer. Further biochemical, biophysical, and mutagenesis data strongly support a Fab-dimerized antibody as the prevalent form that recognizes gp120. The extraordinary configuration of this antibody provides an extended surface, with newly described binding sites, for multivalent interaction with a conserved cluster of oligomannose type sugars on the surface of gp120. The unique interdigitation of Fab domains within an antibody uncovers a previously unappreciated mechanism for high-affinity recognition of carbohydrate or other repeating epitopes on cell or microbial surfaces.

About this Structure

1OP3 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Antibody domain exchange is an immunological solution to carbohydrate cluster recognition., Calarese DA, Scanlan CN, Zwick MB, Deechongkit S, Mimura Y, Kunert R, Zhu P, Wormald MR, Stanfield RL, Roux KH, Kelly JW, Rudd PM, Dwek RA, Katinger H, Burton DR, Wilson IA, Science. 2003 Jun 27;300(5628):2065-71. PMID:12829775

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