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2lai
From Proteopedia
(Difference between revisions)
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| - | + | ==Hyaloperonospora arabidopsidis Effector Protein ATR13== | |
| - | ===Hyaloperonospora | + | <StructureSection load='2lai' size='340' side='right' caption='[[2lai]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[2lai]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hyaloperonospora_parasitica Hyaloperonospora parasitica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LAI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LAI FirstGlance]. <br> | ||
| + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Atr13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=123356 Hyaloperonospora parasitica])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lai OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lai RCSB], [http://www.ebi.ac.uk/pdbsum/2lai PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The oomycete Hyaloperonospora arabidopsidis (Hpa) is the causal agent of downy mildew on the model plant Arabidopsis thaliana and has been adapted as a model system to investigate pathogen virulence strategies and plant disease resistance mechanisms. Recognition of Hpa infection occurs when plant resistance proteins (R-genes) detect the presence or activity of pathogen-derived protein effectors delivered to the plant host. This study examines the Hpa effector ATR13 Emco5 and its recognition by RPP13-Nd, the cognate R-gene that triggers programmed cell death (HR) in the presence of recognized ATR13 variants. Herein, we use NMR to solve the backbone structure of ATR13 Emco5, revealing both a helical domain and a disordered internal loop. Additionally, we use site-directed and random mutagenesis to identify several amino acid residues involved in the recognition response conferred by RPP13-Nd. Using our structure as a scaffold, we map these residues to one of two surface-exposed patches of residues under diversifying selection. Exploring possible roles of the disordered region within the ATR13 structure, we perform domain swapping experiments and identify a peptide sequence involved in nucleolar localization. We conclude that ATR13 is a highly dynamic protein with no clear structural homologues that contains two surface-exposed patches of polymorphism, only one of which is involved in RPP13-Nd recognition specificity. | ||
| - | + | Structural Elucidation and Functional Characterization of the Hyaloperonospora arabidopsidis Effector Protein ATR13.,Leonelli L, Pelton J, Schoeffler A, Dahlbeck D, Berger J, Wemmer DE, Staskawicz B PLoS Pathog. 2011 Dec;7(12):e1002428. Epub 2011 Dec 15. PMID:22194684<ref>PMID:22194684</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Avirulence protein|Avirulence protein]] | *[[Avirulence protein|Avirulence protein]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Hyaloperonospora parasitica]] | [[Category: Hyaloperonospora parasitica]] | ||
| - | [[Category: Leonelli, L | + | [[Category: Leonelli, L]] |
| - | [[Category: Pelton, J G | + | [[Category: Pelton, J G]] |
| - | [[Category: Staskawicz, B J | + | [[Category: Staskawicz, B J]] |
| - | [[Category: Wemmer, D E | + | [[Category: Wemmer, D E]] |
[[Category: Nucleolar localization]] | [[Category: Nucleolar localization]] | ||
[[Category: Signaling protein]] | [[Category: Signaling protein]] | ||
Revision as of 13:44, 17 December 2014
Hyaloperonospora arabidopsidis Effector Protein ATR13
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