This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1f4k
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==CRYSTAL STRUCTURE OF THE REPLICATION TERMINATOR PROTEIN/B-SITE DNA COMPLEX== | |
| - | + | <StructureSection load='1f4k' size='340' side='right' caption='[[1f4k]], [[Resolution|resolution]] 2.50Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[1f4k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F4K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F4K FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bm9|1bm9]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f4k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1f4k RCSB], [http://www.ebi.ac.uk/pdbsum/1f4k PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The coordinated termination of DNA replication is an important step in the life cycle of bacteria with circular chromosomes, but has only been defined at a molecular level in two systems to date. Here we report the structure of an engineered replication terminator protein (RTP) of Bacillus subtilis in complex with a 21 base pair DNA by X-ray crystallography at 2.5 A resolution. We also use NMR spectroscopic titration techniques. This work reveals a novel DNA interaction involving a dimeric 'winged helix' domain protein that differs from predictions. While the two recognition helices of RTP are in close contact with the B-form DNA major grooves, the 'wings' and N-termini of RTP do not form intimate contacts with the DNA. This structure provides insight into the molecular basis of polar replication fork arrest based on a model of cooperative binding and differential binding affinities of RTP to the two adjacent binding sites in the complete terminator. | ||
| - | + | Structure of the RTP-DNA complex and the mechanism of polar replication fork arrest.,Wilce JA, Vivian JP, Hastings AF, Otting G, Folmer RH, Duggin IG, Wake RG, Wilce MC Nat Struct Biol. 2001 Mar;8(3):206-10. PMID:11224562<ref>PMID:11224562</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Bacterial Replication Termination|Bacterial Replication Termination]] | *[[Bacterial Replication Termination|Bacterial Replication Termination]] | ||
| - | *[[RTP and Tus|RTP and Tus]] | ||
*[[Replication Termination Protein|Replication Termination Protein]] | *[[Replication Termination Protein|Replication Termination Protein]] | ||
*[[Tomato aspermy virus protein 2b Suppression of RNA Silencing|Tomato aspermy virus protein 2b Suppression of RNA Silencing]] | *[[Tomato aspermy virus protein 2b Suppression of RNA Silencing|Tomato aspermy virus protein 2b Suppression of RNA Silencing]] | ||
*[[User:Chloe Paul/Replication Terminator Protein|User:Chloe Paul/Replication Terminator Protein]] | *[[User:Chloe Paul/Replication Terminator Protein|User:Chloe Paul/Replication Terminator Protein]] | ||
*[[User:Wayne Decatur/Tomato aspermy virus protein 2b Suppression of RNA Silencing|User:Wayne Decatur/Tomato aspermy virus protein 2b Suppression of RNA Silencing]] | *[[User:Wayne Decatur/Tomato aspermy virus protein 2b Suppression of RNA Silencing|User:Wayne Decatur/Tomato aspermy virus protein 2b Suppression of RNA Silencing]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
| - | [[Category: Duggin, I G | + | [[Category: Duggin, I G]] |
| - | [[Category: Folmer, R H.A | + | [[Category: Folmer, R H.A]] |
| - | [[Category: Hastings, A F | + | [[Category: Hastings, A F]] |
| - | [[Category: Otting, G | + | [[Category: Otting, G]] |
| - | [[Category: Vivian, J P | + | [[Category: Vivian, J P]] |
| - | [[Category: Wake, R G | + | [[Category: Wake, R G]] |
| - | [[Category: Wilce, J A | + | [[Category: Wilce, J A]] |
| - | [[Category: Wilce, M C.J | + | [[Category: Wilce, M C.J]] |
[[Category: Fork arrest mechanism]] | [[Category: Fork arrest mechanism]] | ||
[[Category: Replication and termination]] | [[Category: Replication and termination]] | ||
[[Category: Replication-dna complex]] | [[Category: Replication-dna complex]] | ||
[[Category: Winged-helix protein-dna complex]] | [[Category: Winged-helix protein-dna complex]] | ||
Revision as of 13:51, 17 December 2014
CRYSTAL STRUCTURE OF THE REPLICATION TERMINATOR PROTEIN/B-SITE DNA COMPLEX
| |||||||||||
