2l9s

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
{{STRUCTURE_2l9s| PDB=2l9s | SCENE= }}
+
==Solution structure of Pf1 SID1-mSin3A PAH2 Complex==
-
===Solution structure of Pf1 SID1-mSin3A PAH2 Complex===
+
<StructureSection load='2l9s' size='340' side='right' caption='[[2l9s]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
-
{{ABSTRACT_PUBMED_21440557}}
+
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2l9s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L9S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L9S FirstGlance]. <br>
 +
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1s5q|1s5q]], [[1s5r|1s5r]]</td></tr>
 +
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KIAA1523, Pf1, PHF12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), Kiaa4126, Sin3a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l9s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l9s RCSB], [http://www.ebi.ac.uk/pdbsum/2l9s PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Histone deacetylation constitutes an important mechanism for silencing genes. The histone-deacetylase-associated mammalian Rpd3S/Sin3S corepressor complex plays key roles in repressing aberrant gene transcription from cryptic transcription initiation sites and in mitigating RNA polymerase II progression in intragenic regions of actively transcribed genes. The Sin3 corepressor functions as a molecular adaptor linking histone deacetylases on the one hand, with the chromatin targeting subunits Pf1 and MRG15 on the other. Pf1 also functions as an adaptor by interacting with MRG15 and engaging in multivalent interactions with Sin3 targeting among other domains the two N-terminal paired amphipathic helix (PAH) domains that serve as sites of interaction with sequence-specific DNA-binding transcription factors. Here, we structurally and functionally evaluate the interaction between the PAH2 domain of mSin3A and the Sin3 interaction domain 1 (SID1) motif of Pf1 and find the structural aspects to be reminiscent of the interaction between the Mad1/Mxd1 transcription factor and Sin3. Pf1 residues within a highly conserved sequence motif immediately C-terminal to SID1 appear not to be important for the interaction with Sin3 PAH2. Unexpectedly, the MRG15 subunit competes, rather than collaborates, with Sin3 for the Pf1 segment encompassing the two conserved motifs, implying competition between two subunits for another subunit of the same chromatin-modifying complex.
-
==About this Structure==
+
Solution Structure of the mSin3A PAH2-Pf1 SID1 Complex: A Mad1/Mxd1-Like Interaction Disrupted by MRG15 in the Rpd3S/Sin3S Complex.,Kumar GS, Xie T, Zhang Y, Radhakrishnan I J Mol Biol. 2011 May 20;408(5):987-1000. Epub 2011 Apr 1. PMID:21440557<ref>PMID:21440557</ref>
-
[[2l9s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L9S OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<ref group="xtra">PMID:021440557</ref><references group="xtra"/>
+
</div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
-
[[Category: Kumar, G Senthil.]]
+
[[Category: Kumar, G Senthil]]
-
[[Category: Radhakrishnan, I.]]
+
[[Category: Radhakrishnan, I]]
-
[[Category: Xie, T.]]
+
[[Category: Xie, T]]
-
[[Category: Zhang, Y.]]
+
[[Category: Zhang, Y]]
[[Category: Amphipathic helix motif]]
[[Category: Amphipathic helix motif]]
[[Category: Protein-peptide complex]]
[[Category: Protein-peptide complex]]
[[Category: Transcription]]
[[Category: Transcription]]

Revision as of 14:17, 17 December 2014

Solution structure of Pf1 SID1-mSin3A PAH2 Complex

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools