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3r8f
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| - | + | ==Protein-DNA complex== | |
| - | === | + | <StructureSection load='3r8f' size='340' side='right' caption='[[3r8f]], [[Resolution|resolution]] 3.37Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3r8f]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R8F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R8F FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aq_322, dnaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r8f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r8f RCSB], [http://www.ebi.ac.uk/pdbsum/3r8f PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Many replication initiators form higher-order oligomers that process host replication origins to promote replisome formation. In addition to dedicated duplex-DNA-binding domains, cellular initiators possess AAA+ (ATPases associated with various cellular activities) elements that drive functions ranging from protein assembly to origin recognition. In bacteria, the AAA+ domain of the initiator DnaA has been proposed to assist in single-stranded DNA formation during origin melting. Here we show crystallographically and in solution that the ATP-dependent assembly of Aquifex aeolicus DnaA into a spiral oligomer creates a continuous surface that allows successive AAA+ domains to bind and extend single-stranded DNA segments. The mechanism of binding is unexpectedly similar to that of RecA, a homologous recombination factor, but it differs in that DnaA promotes a nucleic acid conformation that prevents pairing of a complementary strand. These findings, combined with strand-displacement assays, indicate that DnaA opens replication origins by a direct ATP-dependent stretching mechanism. Comparative studies reveal notable commonalities between the approach used by DnaA to engage DNA substrates and other, nucleic-acid-dependent, AAA+ systems. | ||
| - | + | DNA stretching by bacterial initiators promotes replication origin opening.,Duderstadt KE, Chuang K, Berger JM Nature. 2011 Oct 2;478(7368):209-13. doi: 10.1038/nature10455. PMID:21964332<ref>PMID:21964332</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[DnaA|DnaA]] | *[[DnaA|DnaA]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Aquifex aeolicus]] | [[Category: Aquifex aeolicus]] | ||
| - | [[Category: Berger, J M | + | [[Category: Berger, J M]] |
| - | [[Category: Chuang, K | + | [[Category: Chuang, K]] |
| - | [[Category: Duderstadt, K E | + | [[Category: Duderstadt, K E]] |
[[Category: Aaa+ atpase]] | [[Category: Aaa+ atpase]] | ||
[[Category: Dna binding]] | [[Category: Dna binding]] | ||
[[Category: Replication activator-dna complex]] | [[Category: Replication activator-dna complex]] | ||
[[Category: Replication initiator]] | [[Category: Replication initiator]] | ||
Revision as of 08:46, 18 December 2014
Protein-DNA complex
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