1pjy

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[[Image:1pjy.gif|left|200px]]<br /><applet load="1pjy" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1pjy.gif|left|200px]]
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caption="1pjy" />
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'''Solution structure of the HIV-1 frameshift inducing stem-loop RNA'''<br />
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{{Structure
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|PDB= 1pjy |SIZE=350|CAPTION= <scene name='initialview01'>1pjy</scene>
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE=
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}}
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'''Solution structure of the HIV-1 frameshift inducing stem-loop RNA'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1PJY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJY OCA].
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1PJY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJY OCA].
==Reference==
==Reference==
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Solution structure of the HIV-1 frameshift inducing stem-loop RNA., Staple DW, Butcher SE, Nucleic Acids Res. 2003 Aug 1;31(15):4326-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12888491 12888491]
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Solution structure of the HIV-1 frameshift inducing stem-loop RNA., Staple DW, Butcher SE, Nucleic Acids Res. 2003 Aug 1;31(15):4326-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12888491 12888491]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Butcher, S E.]]
[[Category: Butcher, S E.]]
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[[Category: tetraloop]]
[[Category: tetraloop]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:29:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:25:24 2008''

Revision as of 11:25, 20 March 2008

Image:1pjy.gif


PDB ID 1pjy

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Coordinates: save as pdb, mmCIF, xml



Solution structure of the HIV-1 frameshift inducing stem-loop RNA


Overview

The translation of reverse transcriptase and other essential viral proteins from the HIV-1 Pol mRNA requires a programmed -1 ribosomal frameshift. This frameshift is induced by two highly conserved elements within the HIV-1 mRNA: a slippery sequence comprised of a UUUUUUA heptamer, and a downstream stem-loop structure. We have determined the structure of the HIV-1 frameshift inducing RNA stem-loop, using multidimensional heteronuclear nuclear magnetic resonance (NMR) methods. The 22 nucleotide RNA solution structure [root mean squared deviation (r.m.s.d.) = 1.2 A] was determined from 475 nuclear Overhauser effect (NOE)-derived distance restrains, 20 residual dipolar couplings and direct detection of hydrogen bonds via scalar couplings. We find that the frameshift inducing stem-loop is an A-form helix capped by a structured ACAA tetraloop. The ACAA tetraloop is stabilized by an equilateral 5' and 3' stacking pattern, a sheared A-A pair and a cross-strand hydrogen bond. Unexpectedly, the ACAA tetraloop structure is nearly identical to a known tetraloop fold, previously identified in the RNase III recognition site from Saccharomyces cerevisiae.

About this Structure

1PJY is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Solution structure of the HIV-1 frameshift inducing stem-loop RNA., Staple DW, Butcher SE, Nucleic Acids Res. 2003 Aug 1;31(15):4326-31. PMID:12888491

Page seeded by OCA on Thu Mar 20 13:25:24 2008

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