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2lwp

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{{STRUCTURE_2lwp| PDB=2lwp | SCENE= }}
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==The NMR solution structure of the the ubiquitin homology domain of mouse BAG-1==
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===The NMR solution structure of the the ubiquitin homology domain of mouse BAG-1===
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<StructureSection load='2lwp' size='340' side='right' caption='[[2lwp]], [[NMR_Ensembles_of_Models | 18 NMR models]]' scene=''>
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{{ABSTRACT_PUBMED_23277101}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2lwp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LWP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LWP FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Bag1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lwp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lwp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lwp RCSB], [http://www.ebi.ac.uk/pdbsum/2lwp PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BAG-1 (Bcl-2-associated athanogene 1), a multifunctional anti-apoptotic protein known to interact with various cellular proteins, was isolated using its interaction with the anti-apoptotic protein, Bcl-2. A 97-amino acid segment that includes the ubiquitin homology (UBH) domain of mouse BAG-1 (mBAG-1) interacts with a peptide corresponding to the cytoplasmic tail (CT) domain of proHB-EGF. This protein-peptide interaction is likely to have functional significance, as the two species exhibit a synergistic cytoprotective effect. In this study, we determined the solution structure of mBAG-1-UBH and investigated its interaction with the proHB-EGF-CT peptide using isothermal titration calorimetry and NMR spectroscopy. The solution structure of mBAG-1-UBH was shown to be similar to the previously reported structure of hBAG-1-UBH (PDB code 1WXV). However, their electrostatic potential maps demonstrated some differences in the UBH motifs that may be important for protein-peptide interaction. An NMR titration experiment demonstrated that residues 23-26 and residues 89-94 of mBAG-1-UBH are important for its molecular interaction with the peptide proHB-EGF-CT. BAG-1-UBH shares some biological functions with ubiquitin including the formation of polyubiquitin chain and the proteasomal protein degradation. The unique cytoprotective activity suggests mBAG-1-UBH to be an interesting ubiquitin-like protein with distinct biological functions. Here, we first reported the solution structure of mBAG-1-UBH and the growth factor precursor-interacting motif on the protein. For detail understanding about the binding interface and the mechanism of interaction, the study on mBAG-1-UBH/proHB-EGF-CT complex structure is necessary.
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==Function==
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The NMR solution structure of the ubiquitin homology domain of Bcl-2-associated athanogene 1 (BAG-1-UBH) from Mus musculus.,Huang HW, Yu C Biochem Biophys Res Commun. 2013 Feb 1;431(1):86-91. doi:, 10.1016/j.bbrc.2012.12.082. Epub 2012 Dec 28. PMID:23277101<ref>PMID:23277101</ref>
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[[http://www.uniprot.org/uniprot/BAG1_MOUSE BAG1_MOUSE]] Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.<ref>PMID:9873016</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[2lwp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LWP OCA].
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</div>
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==Reference==
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==See Also==
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<ref group="xtra">PMID:023277101</ref><references group="xtra"/><references/>
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*[[BAG protein|BAG protein]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Huang, H.]]
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[[Category: Huang, H]]
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[[Category: Yu, C.]]
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[[Category: Yu, C]]
[[Category: Apoptosis]]
[[Category: Apoptosis]]
[[Category: Proteasomal degradation]]
[[Category: Proteasomal degradation]]

Revision as of 12:49, 18 December 2014

The NMR solution structure of the the ubiquitin homology domain of mouse BAG-1

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