1qet
From Proteopedia
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| - | [[Image:1qet.gif|left|200px]] | + | [[Image:1qet.gif|left|200px]] |
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| - | '''TANDEM GU MISMATCHES IN RNA, NMR, 30 STRUCTURES''' | + | {{Structure |
| + | |PDB= 1qet |SIZE=350|CAPTION= <scene name='initialview01'>1qet</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''TANDEM GU MISMATCHES IN RNA, NMR, 30 STRUCTURES''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1QET is a [ | + | 1QET is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QET OCA]. |
==Reference== | ==Reference== | ||
| - | Investigation of the structural basis for thermodynamic stabilities of tandem GU wobble pairs: NMR structures of (rGGAGUUCC)2 and (rGGAUGUCC)2., McDowell JA, He L, Chen X, Turner DH, Biochemistry. 1997 Jul 1;36(26):8030-8. PMID:[http:// | + | Investigation of the structural basis for thermodynamic stabilities of tandem GU wobble pairs: NMR structures of (rGGAGUUCC)2 and (rGGAUGUCC)2., McDowell JA, He L, Chen X, Turner DH, Biochemistry. 1997 Jul 1;36(26):8030-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9201950 9201950] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Chen, X.]] | [[Category: Chen, X.]] | ||
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[[Category: ribonucleic acid]] | [[Category: ribonucleic acid]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:36:25 2008'' |
Revision as of 11:36, 20 March 2008
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TANDEM GU MISMATCHES IN RNA, NMR, 30 STRUCTURES
Overview
The symmetric, tandem GU mismatch motifs, and , which only differ in the mismatch order, have an average difference in thermodynamic stability of 2 kcal/mol at 37 degrees C. Thermodynamic studies of duplexes containing these motifs indicate the effect is largely localized to the mismatches and adjacent base pairs. The three-dimensional structures of two representative duplexes, (rGGAGUUCC)2 and (rGGAUGUCC)2, were determined by two-dimensional NMR and a simulated annealing protocol. Local deviations are similar to other intrahelical GU mismatches with little effect on backbone torsion angles and a slight overtwisting between the base pair 5' of the G of the mismatch and the mismatch itself. Comparisons of the resulting stacking patterns along with electrostatic potential maps suggest that interactions between highly negative electrostatic regions between base pairs may play a role in the observed thermodynamic differences.
About this Structure
1QET is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Investigation of the structural basis for thermodynamic stabilities of tandem GU wobble pairs: NMR structures of (rGGAGUUCC)2 and (rGGAUGUCC)2., McDowell JA, He L, Chen X, Turner DH, Biochemistry. 1997 Jul 1;36(26):8030-8. PMID:9201950
Page seeded by OCA on Thu Mar 20 13:36:25 2008
