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3oq2

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{{STRUCTURE_3oq2| PDB=3oq2 | SCENE= }}
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==Structure of a CRISPR associated protein Cas2 from Desulfovibrio vulgaris==
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===Structure of a CRISPR associated protein Cas2 from Desulfovibrio vulgaris===
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<StructureSection load='3oq2' size='340' side='right' caption='[[3oq2]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
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{{ABSTRACT_PUBMED_21139194}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3oq2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OQ2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OQ2 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cas2, DVUA0135 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=881 Desulfovibrio vulgaris])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3oq2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oq2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3oq2 RCSB], [http://www.ebi.ac.uk/pdbsum/3oq2 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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CRISPRs (clustered regularly interspaced short palindromic repeats) provide bacteria and archaea with RNA-guided acquired immunity to invasive DNAs. CRISPR-associated (Cas) proteins carry out the immune effector functions. Cas2 is a universal component of the CRISPR system. Here, a 1.35 A resolution crystal structure of Cas2 from the bacterium Desulfovibrio vulgaris (DvuCas2) is reported. DvuCas2 is a homodimer, with each protomer consisting of an N-terminal betaalphabetabetaalphabeta ferredoxin fold (amino acids 1-78) to which is appended a C-terminal segment (amino acids 79-102) that includes a short 3(10)-helix and a fifth beta-strand. The beta5 strands align with the beta4 strands of the opposite protomers, resulting in two five-stranded antiparallel beta-sheets that form a sandwich at the dimer interface. The DvuCas2 dimer is stabilized by a distinctive network of hydrophilic cross-protomer side-chain interactions.
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==Function==
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Structure of a CRISPR-associated protein Cas2 from Desulfovibrio vulgaris.,Samai P, Smith P, Shuman S Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Dec 1;66(Pt, 12):1552-6. Epub 2010 Nov 16. PMID:21139194<ref>PMID:21139194</ref>
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[[http://www.uniprot.org/uniprot/Q72WF4_DESVH Q72WF4_DESVH]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease (By similarity).[HAMAP-Rule:MF_01471]
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3oq2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OQ2 OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:021139194</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Desulfovibrio vulgaris]]
[[Category: Desulfovibrio vulgaris]]
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[[Category: Samai, P.]]
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[[Category: Samai, P]]
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[[Category: Shuman, S.]]
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[[Category: Shuman, S]]
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[[Category: Smith, P.]]
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[[Category: Smith, P]]
[[Category: Crispr]]
[[Category: Crispr]]
[[Category: Ferredoxin fold]]
[[Category: Ferredoxin fold]]
[[Category: Immune system]]
[[Category: Immune system]]

Revision as of 07:16, 19 December 2014

Structure of a CRISPR associated protein Cas2 from Desulfovibrio vulgaris

3oq2, resolution 1.35Å

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