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1r5x

From Proteopedia

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[[Image:1r5x.gif|left|200px]]<br /><applet load="1r5x" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1r5x.gif|left|200px]]
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caption="1r5x, resolution 2.30&Aring;" />
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'''JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome'''<br />
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{{Structure
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|PDB= 1r5x |SIZE=350|CAPTION= <scene name='initialview01'>1r5x</scene>, resolution 2.30&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
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|ACTIVITY=
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|GENE= AF2198 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 Archaeoglobus fulgidus DSM 4304])
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}}
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'''JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1R5X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_dsm_4304 Archaeoglobus fulgidus dsm 4304] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5X OCA].
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1R5X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_dsm_4304 Archaeoglobus fulgidus dsm 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5X OCA].
==Reference==
==Reference==
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JAMM: a metalloprotease-like zinc site in the proteasome and signalosome., Ambroggio XI, Rees DC, Deshaies RJ, PLoS Biol. 2004 Jan;2(1):E2. Epub 2003 Nov 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14737182 14737182]
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JAMM: a metalloprotease-like zinc site in the proteasome and signalosome., Ambroggio XI, Rees DC, Deshaies RJ, PLoS Biol. 2004 Jan;2(1):E2. Epub 2003 Nov 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14737182 14737182]
[[Category: Archaeoglobus fulgidus dsm 4304]]
[[Category: Archaeoglobus fulgidus dsm 4304]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: rpn11]]
[[Category: rpn11]]
[[Category: signalosome]]
[[Category: signalosome]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:47:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:47:17 2008''

Revision as of 11:47, 20 March 2008

Image:1r5x.gif


PDB ID 1r5x

Drag the structure with the mouse to rotate
, resolution 2.30Å
Ligands:
Gene: AF2198 (Archaeoglobus fulgidus DSM 4304)
Coordinates: save as pdb, mmCIF, xml



JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome


Overview

The JAMM (JAB1/MPN/Mov34 metalloenzyme) motif in Rpn11 and Csn5 underlies isopeptidase activities intrinsic to the proteasome and signalosome, respectively. We show here that the archaebacterial protein AfJAMM possesses the key features of a zinc metalloprotease, yet with a distinct fold. The histidine and aspartic acid of the conserved EX(n)HS/THX(7)SXXD motif coordinate a zinc, whereas the glutamic acid hydrogen-bonds an aqua ligand. By analogy to the active site of thermolysin, we predict that the glutamic acid serves as an acid-base catalyst and the second serine stabilizes a tetrahedral intermediate. Mutagenesis of Csn5 confirms these residues are required for Nedd8 isopeptidase activity. The active site-like architecture specified by the JAMM motif motivates structure-based approaches to the study of JAMM domain proteins and the development of therapeutic proteasome and signalosome inhibitors.

About this Structure

1R5X is a Single protein structure of sequence from Archaeoglobus fulgidus dsm 4304. Full crystallographic information is available from OCA.

Reference

JAMM: a metalloprotease-like zinc site in the proteasome and signalosome., Ambroggio XI, Rees DC, Deshaies RJ, PLoS Biol. 2004 Jan;2(1):E2. Epub 2003 Nov 24. PMID:14737182

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