This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3zhf

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
{{STRUCTURE_3zhf| PDB=3zhf | SCENE= }}
+
==gamma 2 adaptin EAR domain crystal structure with preS1 site1 peptide NPDWDFN==
-
===gamma 2 adaptin EAR domain crystal structure with preS1 site1 peptide NPDWDFN===
+
<StructureSection load='3zhf' size='340' side='right' caption='[[3zhf]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
-
{{ABSTRACT_PUBMED_23851574}}
+
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3zhf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZHF FirstGlance]. <br>
 +
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
 +
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zhf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zhf RCSB], [http://www.ebi.ac.uk/pdbsum/3zhf PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Hepatitis B virus (HBV) is an infectious, potentially lethal human pathogen. However, there are no effective therapies for chronic HBV infections. Antiviral development is hampered by the lack of high-resolution structures for essential HBV protein-protein interactions. The interaction between preS1, an HBV surface-protein domain, and its human binding partner, gamma2-adaptin, subverts the membrane-trafficking apparatus to mediate virion export. This interaction is a putative drug target. We report here atomic-resolution descriptions of the binding thermodynamics and structural biology of the interaction between preS1 and the EAR domain of gamma2-adaptin. NMR, protein engineering, X-ray crystallography and MS showed that preS1 contains multiple gamma2-EAR-binding motifs that mimic the membrane-trafficking motifs (and binding modes) of host proteins. These motifs localize together to a relatively rigid, functionally important region of preS1, an intrinsically disordered protein. The preS1-gamma2-EAR interaction was relatively weak and efficiently outcompeted by a synthetic peptide. Our data provide the structural road map for developing peptidomimetic antivirals targeting the gamma2-EAR-preS1 interaction.
-
==Function==
+
The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry.,Jurgens MC, Voros J, Rautureau GJ, Shepherd DA, Pye VE, Muldoon J, Johnson CM, Ashcroft AE, Freund SM, Ferguson N Nat Chem Biol. 2013 Jul 14. doi: 10.1038/nchembio.1294. PMID:23851574<ref>PMID:23851574</ref>
-
[[http://www.uniprot.org/uniprot/AP1G2_HUMAN AP1G2_HUMAN]] May function in protein sorting in late endosomes or multivesucular bodies (MVBs). Involved in MVB-assisted maturation of hepatitis B virus (HBV).<ref>PMID:9733768</ref> <ref>PMID:16867982</ref> <ref>PMID:17553870</ref> [[http://www.uniprot.org/uniprot/Q67953_HBV Q67953_HBV]] The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid (By similarity).[SAAS:SAAS000349_004_055156]
+
-
==About this Structure==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[3zhf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZHF OCA].
+
</div>
==See Also==
==See Also==
*[[Adaptin|Adaptin]]
*[[Adaptin|Adaptin]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:023851574</ref><references group="xtra"/><references/>
+
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Ashcroft, A.]]
+
[[Category: Ashcroft, A]]
-
[[Category: Ferguson, N.]]
+
[[Category: Ferguson, N]]
-
[[Category: Freund, S M.V.]]
+
[[Category: Freund, S M.V]]
-
[[Category: Johnson, C M.]]
+
[[Category: Johnson, C M]]
-
[[Category: Juergens, M C.]]
+
[[Category: Juergens, M C]]
-
[[Category: Muldoon, J.]]
+
[[Category: Muldoon, J]]
-
[[Category: Pye, V E.]]
+
[[Category: Pye, V E]]
-
[[Category: Rautureau, G.]]
+
[[Category: Rautureau, G]]
-
[[Category: Shepherd, D.]]
+
[[Category: Shepherd, D]]
-
[[Category: Voros, J.]]
+
[[Category: Voros, J]]
[[Category: Gae]]
[[Category: Gae]]
[[Category: Protein transport-viral protein complex]]
[[Category: Protein transport-viral protein complex]]

Revision as of 09:43, 21 December 2014

gamma 2 adaptin EAR domain crystal structure with preS1 site1 peptide NPDWDFN

3zhf, resolution 1.70Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3zhf"
Personal tools