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4fl0

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{{STRUCTURE_4fl0| PDB=4fl0 | SCENE= }}
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==Crystal structure of ALD1 from Arabidopsis thaliana==
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===Crystal structure of ALD1 from Arabidopsis thaliana===
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<StructureSection load='4fl0' size='340' side='right' caption='[[4fl0]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23385743}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4fl0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FL0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FL0 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ALD1, At2g13810, F13J11.16 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fl0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fl0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fl0 RCSB], [http://www.ebi.ac.uk/pdbsum/4fl0 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Diaminopimelate aminotransferase (DAP-AT) is an enzyme in the lysine-biosynthesis pathway. Conversely, ALD1, a close homologue of DAP-AT in plants, uses lysine as a substrate in vitro. Both proteins require pyridoxal-5'-phosphate (PLP) for their activity. The structure of ALD1 from the flowering plant Arabidopsis thaliana (AtALD1) was solved at a resolution of 2.3 A. Comparison of AtALD1 with the previously solved structure of A. thaliana DAP-AT (AtDAP-AT) revealed similar interactions with PLP despite sequence differences within the PLP-binding site. However, sequence differences between the binding site of AtDAP-AT for malate, a purported mimic of substrate binding, and the corresponding site in AtALD1 led to different interactions. This suggests that either the substrate itself, or the substrate-binding mode, differs in the two proteins, supporting the known in vitro findings.
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==Function==
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Structure of ALD1, a plant-specific homologue of the universal diaminopimelate aminotransferase enzyme of lysine biosynthesis.,Sobolev V, Edelman M, Dym O, Unger T, Albeck S, Kirma M, Galili G Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Feb 1;69(Pt 2):84-9. doi: , 10.1107/S1744309112050270. Epub 2013 Jan 26. PMID:23385743<ref>PMID:23385743</ref>
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[[http://www.uniprot.org/uniprot/ALD1_ARATH ALD1_ARATH]] Aminotransferase involved in local and systemic acquired resistance (SAR) to the bacterial pathogen P.syringae. Required for salicylic acid (SA) and camalexin accumulation upon pathogen infection. Possesses aminotransferase activity in vitro and may generate amino-acid-derived defense signals in vivo. May be involved in ethylene-induced senescence signaling.<ref>PMID:14729919</ref> <ref>PMID:15447647</ref> <ref>PMID:19825557</ref> <ref>PMID:18266921</ref> <ref>PMID:21530897</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4fl0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FL0 OCA].
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</div>
==See Also==
==See Also==
*[[Journal:Acta Cryst F:2|Journal:Acta Cryst F:2]]
*[[Journal:Acta Cryst F:2|Journal:Acta Cryst F:2]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:023385743</ref><ref group="xtra">PMID:000000000</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
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[[Category: Albeck, S.]]
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[[Category: Albeck, S]]
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[[Category: Dym, O.]]
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[[Category: Dym, O]]
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[[Category: Edelman, M.]]
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[[Category: Edelman, M]]
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[[Category: Galili, G.]]
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[[Category: Galili, G]]
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[[Category: ISPC, Israel Structural Proteomics Center.]]
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[[Category: ISPC, Israel Structural Proteomics Center]]
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[[Category: Kirma, M.]]
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[[Category: Kirma, M]]
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[[Category: Sobolev, V.]]
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[[Category: Sobolev, V]]
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[[Category: Unger, T.]]
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[[Category: Unger, T]]
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[[Category: ISPC]]
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[[Category: Israel Structural Proteomics Center]]
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[[Category: Pathogen resistance]]
[[Category: Pathogen resistance]]
[[Category: Plp nbinding]]
[[Category: Plp nbinding]]
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[[Category: Protein structure initiative]]
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[[Category: PSI, Protein structure initiative]]
[[Category: Sandwich fold and architecture]]
[[Category: Sandwich fold and architecture]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Transferase]]
[[Category: Transferase]]

Revision as of 10:39, 21 December 2014

Crystal structure of ALD1 from Arabidopsis thaliana

4fl0, resolution 2.30Å

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