1s0v

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[[Image:1s0v.gif|left|200px]]<br /><applet load="1s0v" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1s0v.gif|left|200px]]
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caption="1s0v, resolution 3.2&Aring;" />
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'''Structural basis for substrate selection by T7 RNA polymerase'''<br />
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{{Structure
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|PDB= 1s0v |SIZE=350|CAPTION= <scene name='initialview01'>1s0v</scene>, resolution 3.2&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER'>APC</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6]
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|GENE= 1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Bacteriophage T7])
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}}
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'''Structural basis for substrate selection by T7 RNA polymerase'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1S0V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=APC:'>APC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S0V OCA].
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1S0V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S0V OCA].
==Reference==
==Reference==
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Structural basis for substrate selection by t7 RNA polymerase., Temiakov D, Patlan V, Anikin M, McAllister WT, Yokoyama S, Vassylyev DG, Cell. 2004 Feb 6;116(3):381-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15016373 15016373]
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Structural basis for substrate selection by t7 RNA polymerase., Temiakov D, Patlan V, Anikin M, McAllister WT, Yokoyama S, Vassylyev DG, Cell. 2004 Feb 6;116(3):381-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15016373 15016373]
[[Category: Bacteriophage t7]]
[[Category: Bacteriophage t7]]
[[Category: DNA-directed RNA polymerase]]
[[Category: DNA-directed RNA polymerase]]
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[[Category: rna]]
[[Category: rna]]
[[Category: rsgi]]
[[Category: rsgi]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
[[Category: t7 rna polymerase]]
[[Category: t7 rna polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:56:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:58:55 2008''

Revision as of 11:58, 20 March 2008

Image:1s0v.gif


PDB ID 1s0v

Drag the structure with the mouse to rotate
, resolution 3.2Å
Ligands: and
Gene: 1 (Bacteriophage T7)
Activity: DNA-directed RNA polymerase, with EC number 2.7.7.6
Coordinates: save as pdb, mmCIF, xml



Structural basis for substrate selection by T7 RNA polymerase


Overview

The mechanism by which nucleotide polymerases select the correct substrate is of fundamental importance to the fidelity of DNA replication and transcription. During the nucleotide addition cycle, pol I DNA polymerases undergo the transition from a catalytically inactive "open" to an active "closed" conformation. All known determinants of substrate selection are associated with the "closed" state. To elucidate if this mechanism is conserved in homologous single subunit RNA polymerases (RNAPs), we have determined the structure of T7 RNAP elongation complex with the incoming substrate analog. Surprisingly, the substrate specifically binds to RNAP in the "open" conformation, where it is base paired with the acceptor template base, while Tyr639 provides discrimination of ribose versus deoxyribose substrates. The structure therefore suggests a novel mechanism, in which the substrate selection occurs prior to the isomerization to the catalytically active conformation. Modeling of multisubunit RNAPs suggests that this mechanism might be universal for all RNAPs.

About this Structure

1S0V is a Single protein structure of sequence from Bacteriophage t7. Full crystallographic information is available from OCA.

Reference

Structural basis for substrate selection by t7 RNA polymerase., Temiakov D, Patlan V, Anikin M, McAllister WT, Yokoyama S, Vassylyev DG, Cell. 2004 Feb 6;116(3):381-91. PMID:15016373

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