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4j8s

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{{STRUCTURE_4j8s| PDB=4j8s | SCENE= }}
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==Crystal structure of human CNOT1 MIF4G domain in complex with a TTP peptide==
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===Crystal structure of human CNOT1 MIF4G domain in complex with a TTP peptide===
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<StructureSection load='4j8s' size='340' side='right' caption='[[4j8s]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23644599}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4j8s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J8S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J8S FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AD-005, CDC39, CNOT1, KIAA1007, NOT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j8s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j8s RCSB], [http://www.ebi.ac.uk/pdbsum/4j8s PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Tristetraprolin (TTP) is an RNA-binding protein that controls the inflammatory response by limiting the expression of several proinflammatory cytokines. TTP post-transcriptionally represses gene expression by interacting with AU-rich elements (AREs) in 3' untranslated regions of target mRNAs and subsequently engenders their deadenylation and decay. TTP accomplishes these tasks, at least in part, by recruiting the multisubunit CCR4-NOT deadenylase complex to the mRNA. Here we identify an evolutionarily conserved C-terminal motif in human TTP that directly binds a central domain of CNOT1, a core subunit of the CCR4-NOT complex. A high-resolution crystal structure of the TTP-CNOT1 complex was determined, providing the first structural insight, to our knowledge, into an ARE-binding protein bound to the CCR4-NOT complex. Mutations at the CNOT1-TTP interface impair TTP-mediated deadenylation, demonstrating the significance of this interaction in TTP-mediated gene silencing.
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==Function==
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Structural basis for the recruitment of the human CCR4-NOT deadenylase complex by tristetraprolin.,Fabian MR, Frank F, Rouya C, Siddiqui N, Lai WS, Karetnikov A, Blackshear PJ, Nagar B, Sonenberg N Nat Struct Mol Biol. 2013 Jun;20(6):735-9. doi: 10.1038/nsmb.2572. Epub 2013 May , 5. PMID:23644599<ref>PMID:23644599</ref>
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[[http://www.uniprot.org/uniprot/CNOT1_HUMAN CNOT1_HUMAN]] Belongs to the CCR4-NOT complex that functions as general transcription regulation complex. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors.<ref>PMID:10637334</ref> <ref>PMID:16778766</ref> [[http://www.uniprot.org/uniprot/TTP_HUMAN TTP_HUMAN]] mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. Acts by specifically binding ARE-containing mRNAs and promoting their degradation. Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF).<ref>PMID:15014438</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4j8s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J8S OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:023644599</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Blackshear, P J.]]
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[[Category: Blackshear, P J]]
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[[Category: Fabian, M R.]]
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[[Category: Fabian, M R]]
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[[Category: Frank, F.]]
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[[Category: Frank, F]]
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[[Category: Karetnikov, A.]]
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[[Category: Karetnikov, A]]
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[[Category: Lai, W S.]]
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[[Category: Lai, W S]]
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[[Category: Nagar, B.]]
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[[Category: Nagar, B]]
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[[Category: Rouya, C.]]
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[[Category: Rouya, C]]
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[[Category: Siddiqui, N.]]
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[[Category: Siddiqui, N]]
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[[Category: Sonenberg, N.]]
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[[Category: Sonenberg, N]]
[[Category: Cytosol]]
[[Category: Cytosol]]
[[Category: Mif4g]]
[[Category: Mif4g]]

Revision as of 12:37, 21 December 2014

Crystal structure of human CNOT1 MIF4G domain in complex with a TTP peptide

4j8s, resolution 1.55Å

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