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4imk

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{{STRUCTURE_4imk| PDB=4imk | SCENE= }}
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==Uncrossed Fab binding to human Angiopoietin 2==
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===Uncrossed Fab binding to human Angiopoietin 2===
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<StructureSection load='4imk' size='340' side='right' caption='[[4imk]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23613981}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4imk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IMK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IMK FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4iml|4iml]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4imk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4imk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4imk RCSB], [http://www.ebi.ac.uk/pdbsum/4imk PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bispecific antibodies are considered as a promising class of future biotherapeutic molecules. They comprise binding specificities for two different antigens, which may provide additive or synergistic modes of action. There is a wide variety of design alternatives for such bispecific antibodies, including the "CrossMab" format. CrossMabs contain a domain crossover in one of the antigen-binding (Fab) parts, together with the "knobs-and-holes" approach, to enforce the correct assembly of four different polypeptide chains into an IgG-like bispecific antibody. We determined the crystal structure of a hAng-2-binding Fab in its crossed and uncrossed form and show that CH1-CL-domain crossover does not induce significant perturbations of the structure and has no detectable influence on target binding.
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==About this Structure==
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Crystal structure of an anti-Ang2 CrossFab demonstrates complete structural and functional integrity of the variable domain.,Fenn S, Schiller CB, Griese JJ, Duerr H, Imhof-Jung S, Gassner C, Moelleken J, Regula JT, Schaefer W, Thomas M, Klein C, Hopfner KP, Kettenberger H PLoS One. 2013 Apr 17;8(4):e61953. doi: 10.1371/journal.pone.0061953. Print 2013. PMID:23613981<ref>PMID:23613981</ref>
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[[4imk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IMK OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023613981</ref><references group="xtra"/><references/>
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</div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Fenn, S.]]
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[[Category: Fenn, S]]
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[[Category: Griese, J J.]]
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[[Category: Griese, J J]]
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[[Category: Hopfner, K P.]]
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[[Category: Hopfner, K P]]
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[[Category: Kettenberger, H.]]
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[[Category: Kettenberger, H]]
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[[Category: Schiller, C.]]
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[[Category: Schiller, C]]
[[Category: Human angiopoietin 2]]
[[Category: Human angiopoietin 2]]
[[Category: Immune system]]
[[Category: Immune system]]

Revision as of 12:42, 21 December 2014

Uncrossed Fab binding to human Angiopoietin 2

4imk, resolution 2.20Å

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