1s6r
From Proteopedia
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| - | [[Image:1s6r.gif|left|200px]] | + | [[Image:1s6r.gif|left|200px]] |
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| - | '''908R CLASS C BETA-LACTAMASE BOUND TO IODO-ACETAMIDO-PHENYL BORONIC ACID''' | + | {{Structure |
| + | |PDB= 1s6r |SIZE=350|CAPTION= <scene name='initialview01'>1s6r</scene>, resolution 2.24Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=IAP:4-IODO-ACETAMIDO PHENYLBORONIC ACID'>IAP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6. 3.5.2.6.] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''908R CLASS C BETA-LACTAMASE BOUND TO IODO-ACETAMIDO-PHENYL BORONIC ACID''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1S6R is a [ | + | 1S6R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S6R OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of Enterobacter cloacae 908R class C beta-lactamase bound to iodo-acetamido-phenyl boronic acid, a transition-state analogue., Wouters J, Fonze E, Vermeire M, Frere JM, Charlier P, Cell Mol Life Sci. 2003 Aug;60(8):1764-73. PMID:[http:// | + | Crystal structure of Enterobacter cloacae 908R class C beta-lactamase bound to iodo-acetamido-phenyl boronic acid, a transition-state analogue., Wouters J, Fonze E, Vermeire M, Frere JM, Charlier P, Cell Mol Life Sci. 2003 Aug;60(8):1764-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14521155 14521155] |
[[Category: Enterobacter cloacae]] | [[Category: Enterobacter cloacae]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:01:11 2008'' |
Revision as of 12:01, 20 March 2008
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| , resolution 2.24Å | |||||||
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| Ligands: | |||||||
| Activity: | Hydrolase, with EC number 3.5.2.6. | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
908R CLASS C BETA-LACTAMASE BOUND TO IODO-ACETAMIDO-PHENYL BORONIC ACID
Overview
The structures of the class C beta-lactamase from Enterobacter cloacae 908R alone and in complex with a boronic acid transition-state analogue were determined by X-ray crystallography at 2.1 and 2.3 A, respectively. The structure of the enzyme resembles those of other class C beta-lactamases. The structure of the complex with the transition-state analogue, iodo-acetamido-phenyl boronic acid, shows that the inhibitor is covalently bound to the active-site serine (Ser64). Binding of the inhibitor within the active site is compared with previously determined structures of complexes with other class C enzymes. The structure of the boronic acid adduct indicates ways to improve the affinity of this class of inhibitors. This structure of 908R class C beta-lactamase in complex with a transition-state analogue provides further insights into the mechanism of action of these hydrolases.
About this Structure
1S6R is a Single protein structure of sequence from Enterobacter cloacae. Full crystallographic information is available from OCA.
Reference
Crystal structure of Enterobacter cloacae 908R class C beta-lactamase bound to iodo-acetamido-phenyl boronic acid, a transition-state analogue., Wouters J, Fonze E, Vermeire M, Frere JM, Charlier P, Cell Mol Life Sci. 2003 Aug;60(8):1764-73. PMID:14521155
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