1s9h
From Proteopedia
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| - | [[Image:1s9h.gif|left|200px]] | + | [[Image:1s9h.gif|left|200px]] |
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| - | '''Crystal Structure of Adeno-associated virus Type 2 Rep40''' | + | {{Structure |
| + | |PDB= 1s9h |SIZE=350|CAPTION= <scene name='initialview01'>1s9h</scene>, resolution 2.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= REP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= adeno-associated virus 2]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of Adeno-associated virus Type 2 Rep40''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1S9H is a [ | + | 1S9H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_2 Adeno-associated virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9H OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the SF3 helicase from adeno-associated virus type 2., James JA, Escalante CR, Yoon-Robarts M, Edwards TA, Linden RM, Aggarwal AK, Structure. 2003 Aug;11(8):1025-35. PMID:[http:// | + | Crystal structure of the SF3 helicase from adeno-associated virus type 2., James JA, Escalante CR, Yoon-Robarts M, Edwards TA, Linden RM, Aggarwal AK, Structure. 2003 Aug;11(8):1025-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12906833 12906833] |
[[Category: Adeno-associated virus 2]] | [[Category: Adeno-associated virus 2]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: p-loop]] | [[Category: p-loop]] | ||
[[Category: sensor 1]] | [[Category: sensor 1]] | ||
| - | [[Category: walker | + | [[Category: walker some]] |
[[Category: walker b]] | [[Category: walker b]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:02:05 2008'' |
Revision as of 12:02, 20 March 2008
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| , resolution 2.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | REP (adeno-associated virus 2) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Adeno-associated virus Type 2 Rep40
Overview
We report here the crystal structure of an SF3 DNA helicase, Rep40, from adeno-associated virus 2 (AAV2). We show that AAV2 Rep40 is structurally more similar to the AAA(+) class of cellular proteins than to DNA helicases from other superfamilies. The structure delineates the expected Walker A and B motifs, but also reveals an unexpected "arginine finger" that directly implies the requirement of Rep40 oligomerization for ATP hydrolysis and helicase activity. Further, the Rep40 AAA(+) domain is novel in that it is unimodular as opposed to bimodular. Altogether, the structural connection to AAA(+) proteins defines the general architecture of SF3 DNA helicases, a family that includes simian virus 40 (SV40) T antigen, as well as provides a conceptual framework for understanding the role of Rep proteins during AAV DNA replication, packaging, and site-specific integration.
About this Structure
1S9H is a Single protein structure of sequence from Adeno-associated virus 2. Full crystallographic information is available from OCA.
Reference
Crystal structure of the SF3 helicase from adeno-associated virus type 2., James JA, Escalante CR, Yoon-Robarts M, Edwards TA, Linden RM, Aggarwal AK, Structure. 2003 Aug;11(8):1025-35. PMID:12906833
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