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4k1c
From Proteopedia
(Difference between revisions)
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| - | + | ==VCX1 Calcium/Proton Exchanger== | |
| - | ===VCX1 | + | <StructureSection load='4k1c' size='340' side='right' caption='[[4k1c]], [[Resolution|resolution]] 2.30Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4k1c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K1C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K1C FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VCX1, HUM1, MNR1, YDL128W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k1c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k1c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k1c RCSB], [http://www.ebi.ac.uk/pdbsum/4k1c PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Eukaryotic Ca2+ regulation involves sequestration into intracellular organelles, and expeditious Ca2+ release into the cytosol is a hallmark of key signalling transduction pathways. Bulk removal of Ca2+ after such signalling events is accomplished by members of the Ca2+:cation (CaCA) superfamily. The CaCA superfamily includes the Na+/Ca2+ (NCX) and Ca2+/H+ (CAX) antiporters, and in mammals the NCX and related proteins constitute families SLC8 and SLC24, and are responsible for the re-establishment of Ca2+ resting potential in muscle cells, neuronal signalling and Ca2+ reabsorption in the kidney. The CAX family members maintain cytosolic Ca2+ homeostasis in plants and fungi during steep rises in intracellular Ca2+ due to environmental changes, or following signal transduction caused by events such as hyperosmotic shock, hormone response and response to mating pheromones. The cytosol-facing conformations within the CaCA superfamily are unknown, and the transport mechanism remains speculative. Here we determine a crystal structure of the Saccharomyces cerevisiae vacuolar Ca2+/H+ exchanger (Vcx1) at 2.3 A resolution in a cytosol-facing, substrate-bound conformation. Vcx1 is the first structure, to our knowledge, within the CAX family, and it describes the key cytosol-facing conformation of the CaCA superfamily, providing the structural basis for a novel alternating access mechanism by which the CaCA superfamily performs high-throughput Ca2+ transport across membranes. | ||
| - | + | Structural basis for alternating access of a eukaryotic calcium/proton exchanger.,Waight AB, Pedersen BP, Schlessinger A, Bonomi M, Chau BH, Roe-Zurz Z, Risenmay AJ, Sali A, Stroud RM Nature. 2013 May 19. doi: 10.1038/nature12233. PMID:23685453<ref>PMID:23685453</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Saccharomyces cerevisiae s288c]] | [[Category: Saccharomyces cerevisiae s288c]] | ||
| - | [[Category: CSMP, Center for Structures of Membrane Proteins | + | [[Category: CSMP, Center for Structures of Membrane Proteins]] |
| - | [[Category: Pedersen, B P | + | [[Category: Pedersen, B P]] |
| - | [[Category: Stroud, R M | + | [[Category: Stroud, R M]] |
| - | [[Category: Waight, A B | + | [[Category: Waight, A B]] |
[[Category: Calcium transport]] | [[Category: Calcium transport]] | ||
[[Category: Center for structures of membrane protein]] | [[Category: Center for structures of membrane protein]] | ||
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[[Category: Membrane protein cax ncx nckx]] | [[Category: Membrane protein cax ncx nckx]] | ||
[[Category: Membrane protein-metal transport complex]] | [[Category: Membrane protein-metal transport complex]] | ||
| - | [[Category: Protein structure initiative]] | + | [[Category: PSI, Protein structure initiative]] |
[[Category: Psi-biology]] | [[Category: Psi-biology]] | ||
[[Category: Structural genomic]] | [[Category: Structural genomic]] | ||
[[Category: Transport protein]] | [[Category: Transport protein]] | ||
[[Category: Vacuole]] | [[Category: Vacuole]] | ||
Revision as of 14:51, 21 December 2014
VCX1 Calcium/Proton Exchanger
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Categories: Saccharomyces cerevisiae s288c | CSMP, Center for Structures of Membrane Proteins | Pedersen, B P | Stroud, R M | Waight, A B | Calcium transport | Center for structures of membrane protein | Csmp | Membrane protein cax ncx nckx | Membrane protein-metal transport complex | PSI, Protein structure initiative | Psi-biology | Structural genomic | Transport protein | Vacuole
