This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1sty
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1sty.jpg|left|200px]] | + | [[Image:1sty.jpg|left|200px]] |
| - | + | ||
| - | '''THE ALPHA ANEURISM: A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE''' | + | {{Structure |
| + | |PDB= 1sty |SIZE=350|CAPTION= <scene name='initialview01'>1sty</scene>, resolution 1.67Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=THP:THYMIDINE-3',5'-DIPHOSPHATE'>THP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Micrococcal_nuclease Micrococcal nuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.31.1 3.1.31.1] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''THE ALPHA ANEURISM: A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1STY is a [ | + | 1STY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1STY OCA]. |
==Reference== | ==Reference== | ||
| - | The alpha aneurism: a structural motif revealed in an insertion mutant of staphylococcal nuclease., Keefe LJ, Sondek J, Shortle D, Lattman EE, Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3275-9. PMID:[http:// | + | The alpha aneurism: a structural motif revealed in an insertion mutant of staphylococcal nuclease., Keefe LJ, Sondek J, Shortle D, Lattman EE, Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3275-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8475069 8475069] |
[[Category: Micrococcal nuclease]] | [[Category: Micrococcal nuclease]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 20: | Line 29: | ||
[[Category: hydrolase (phosphoric diester)]] | [[Category: hydrolase (phosphoric diester)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:09:34 2008'' |
Revision as of 12:09, 20 March 2008
| |||||||
| , resolution 1.67Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | Micrococcal nuclease, with EC number 3.1.31.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE ALPHA ANEURISM: A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE
Overview
The x-ray crystal structure of a mutant of staphylococcal nuclease that contains a single glycine residue inserted in the C-terminal alpha-helix has been solved to 1.67 A resolution and refined to a crystallographic R value of 0.170. This inserted glycine residue is accommodated in the alpha-helix by formation of a previously uncharacterized bulge, which we term the alpha aneurism. A conformational search of known protein structures has identified the alpha aneurism in a number of protein families, including the histocompatibility antigens and hemoglobins.
About this Structure
1STY is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
Reference
The alpha aneurism: a structural motif revealed in an insertion mutant of staphylococcal nuclease., Keefe LJ, Sondek J, Shortle D, Lattman EE, Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3275-9. PMID:8475069
Page seeded by OCA on Thu Mar 20 14:09:34 2008
