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2m39

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{{STRUCTURE_2m39| PDB=2m39 | SCENE= }}
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==The solution structure of 3',5'-LINKED 2'-O-(2-METHOXYETHYL)-RNA DUPLEX==
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===The solution structure of 3',5'-LINKED 2'-O-(2-METHOXYETHYL)-RNA DUPLEX===
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<StructureSection load='2m39' size='340' side='right' caption='[[2m39]], [[NMR_Ensembles_of_Models | 9 NMR models]]' scene=''>
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{{ABSTRACT_PUBMED_24012551}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2m39]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M39 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2M39 FirstGlance]. <br>
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A44:2-O-METHYOXYETHYL-ADENOSINE+5-MONOPHOSPHATE'>A44</scene>, <scene name='pdbligand=C5L:2-O-(2-METHOXYETHYL)-5-METHYLCYTIDINE+5-(DIHYDROGEN+PHOSPHATE)'>C5L</scene>, <scene name='pdbligand=G48:2-O-METHYOXYETHYL-GUANOSINE-5-MONOPHOSPHATE'>G48</scene>, <scene name='pdbligand=T39:2-O-METHOXYETHYLENE+THYMIDINE+5-MONOPHOSPHATE'>T39</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m39 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2m39 RCSB], [http://www.ebi.ac.uk/pdbsum/2m39 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The solution-state structure of 2'-O-(2-methoxyethly) substituted dodecamer r(*CG*CGAA*U*U*CG*C)d(G), 2'-MOE RNA, with all cytosines and uracils methylated at the C5-position has been determined by NMR spectroscopy. The chemical modifications were used to improve the oligonucleotide's drug-like properties. The 2'-MOE group drives pseudorotational equilibrium of the ribofuranose moiety to the N-type conformation and supposedly results in structural preorganization leading to high affinity of a modified oligonucleotide towards its complementary biological target, improved pharmacokinetic and toxicological properties. The high melting temperature of the antiparallel duplex structure adopted by 2'-MOE RNA was explained through the formation of a stable A-form RNA consistent with effective base-pairing and stacking interactions. The comparison of the solution-state structure with the crystal structure of a non-methylated analogue shows an increase in the stacking at the base pair steps for the C5-methylated 2'-MOE RNA duplex. The MOE substituents adopt a well-defined structure in the minor groove with the predominant gauche conformations around the ethylene bond.
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==About this Structure==
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NMR structure of 2'-O-(2-methoxyethyl) modified and C5-methylated RNA dodecamer duplex.,Plevnik M, Cevec M, Plavec J Biochimie. 2013 Dec;95(12):2385-91. doi: 10.1016/j.biochi.2013.08.025. Epub 2013 , Sep 3. PMID:24012551<ref>PMID:24012551</ref>
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[[2m39]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M39 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:024012551</ref><references group="xtra"/><references/>
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</div>
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[[Category: Cevec, M.]]
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== References ==
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[[Category: Plavec, J.]]
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<references/>
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[[Category: Plevnik, M.]]
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__TOC__
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</StructureSection>
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[[Category: Cevec, M]]
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[[Category: Plavec, J]]
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[[Category: Plevnik, M]]
[[Category: Duplex]]
[[Category: Duplex]]
[[Category: Rna]]
[[Category: Rna]]

Revision as of 18:13, 21 December 2014

The solution structure of 3',5'-LINKED 2'-O-(2-METHOXYETHYL)-RNA DUPLEX

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