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1t44
From Proteopedia
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| - | [[Image:1t44.gif|left|200px]] | + | [[Image:1t44.gif|left|200px]] |
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| - | '''Structural basis of actin sequestration by thymosin-B4: Implications for arp2/3 activation''' | + | {{Structure |
| + | |PDB= 1t44 |SIZE=350|CAPTION= <scene name='initialview01'>1t44</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=ATP:ADENOSINE-5'-TRIPHOSPHATE'>ATP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Structural basis of actin sequestration by thymosin-B4: Implications for arp2/3 activation''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1T44 is a [ | + | 1T44 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens,_mus_musculus Homo sapiens, mus musculus] and [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T44 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis of actin sequestration by thymosin-beta4: implications for WH2 proteins., Irobi E, Aguda AH, Larsson M, Guerin C, Yin HL, Burtnick LD, Blanchoin L, Robinson RC, EMBO J. 2004 Sep 15;23(18):3599-608. Epub 2004 Aug 26. PMID:[http:// | + | Structural basis of actin sequestration by thymosin-beta4: implications for WH2 proteins., Irobi E, Aguda AH, Larsson M, Guerin C, Yin HL, Burtnick LD, Blanchoin L, Robinson RC, EMBO J. 2004 Sep 15;23(18):3599-608. Epub 2004 Aug 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15329672 15329672] |
[[Category: Homo sapiens, mus musculus]] | [[Category: Homo sapiens, mus musculus]] | ||
[[Category: Oryctolagus cuniculus]] | [[Category: Oryctolagus cuniculus]] | ||
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[[Category: structural protein]] | [[Category: structural protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:13:30 2008'' |
Revision as of 12:13, 20 March 2008
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| , resolution 2.00Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structural basis of actin sequestration by thymosin-B4: Implications for arp2/3 activation
Contents |
Overview
The WH2 (Wiscott-Aldridge syndrome protein homology domain 2) repeat is an actin interacting motif found in monomer sequestering and filament assembly proteins. We have stabilized the prototypical WH2 family member, thymosin-beta4 (Tbeta4), with respect to actin, by creating a hybrid between gelsolin domain 1 and the C-terminal half of Tbeta4 (G1-Tbeta4). This hybrid protein sequesters actin monomers, severs actin filaments and acts as a leaky barbed end cap. Here, we present the structure of the G1-Tbeta4:actin complex at 2 A resolution. The structure reveals that Tbeta4 sequesters by capping both ends of the actin monomer, and that exchange of actin between Tbeta4 and profilin is mediated by a minor overlap in binding sites. The structure implies that multiple WH2 motif-containing proteins will associate longitudinally with actin filaments. Finally, we discuss the role of the WH2 motif in arp2/3 activation.
Disease
Known diseases associated with this structure: Myopathy, actin, congenital, with cores OMIM:[102610], Myopathy, actin, congenital, with excess of thin myofilaments OMIM:[102610], Myopathy, congenital, with fiber-type disporportion 1 OMIM:[102610], Myopathy, nemaline, 3 OMIM:[102610]
About this Structure
1T44 is a Protein complex structure of sequences from Homo sapiens, mus musculus and Oryctolagus cuniculus. Full crystallographic information is available from OCA.
Reference
Structural basis of actin sequestration by thymosin-beta4: implications for WH2 proteins., Irobi E, Aguda AH, Larsson M, Guerin C, Yin HL, Burtnick LD, Blanchoin L, Robinson RC, EMBO J. 2004 Sep 15;23(18):3599-608. Epub 2004 Aug 26. PMID:15329672
Page seeded by OCA on Thu Mar 20 14:13:30 2008
