1t94
From Proteopedia
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| - | [[Image:1t94.gif|left|200px]] | + | [[Image:1t94.gif|left|200px]] |
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| - | '''Crystal structure of the catalytic core of human DNA polymerase kappa''' | + | {{Structure |
| + | |PDB= 1t94 |SIZE=350|CAPTION= <scene name='initialview01'>1t94</scene>, resolution 2.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= DINB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the catalytic core of human DNA polymerase kappa''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1T94 is a [ | + | 1T94 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T94 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the catalytic core of human DNA polymerase kappa., Uljon SN, Johnson RE, Edwards TA, Prakash S, Prakash L, Aggarwal AK, Structure. 2004 Aug;12(8):1395-404. PMID:[http:// | + | Crystal structure of the catalytic core of human DNA polymerase kappa., Uljon SN, Johnson RE, Edwards TA, Prakash S, Prakash L, Aggarwal AK, Structure. 2004 Aug;12(8):1395-404. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15296733 15296733] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: replication; dna repair; y-family dna polymerase; translesion dna synthesis; lesion bypass]] | [[Category: replication; dna repair; y-family dna polymerase; translesion dna synthesis; lesion bypass]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:15:34 2008'' |
Revision as of 12:15, 20 March 2008
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| , resolution 2.40Å | |||||||
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| Gene: | DINB (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the catalytic core of human DNA polymerase kappa
Overview
We present the crystal structure of the catalytic core of human DNA polymerase kappa (hPolkappa), the first structure of a human Y-family polymerase. hPolkappa is implicated in the proficient extension of mispaired primer termini on undamaged DNAs, and in the extension step of lesion bypass. The structure reveals a stubby "fingers" subdomain, which despite its small size appears to be tightly restrained with respect to a putative templating base. The structure also reveals a novel "thumb" subdomain that provides a basis for the importance of the N-terminal extension unique to hPolkappa. And, most surprisingly, the structure reveals the polymerase-associated domain (PAD) juxtaposed on the dorsal side of the "palm" subdomain, as opposed to the fingers subdomain. Together, these properties suggest that the hPolkappa active site is constrained at the site of the templating base and incoming nucleotide, but the polymerase is less constrained following translocation of the lesion.
About this Structure
1T94 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the catalytic core of human DNA polymerase kappa., Uljon SN, Johnson RE, Edwards TA, Prakash S, Prakash L, Aggarwal AK, Structure. 2004 Aug;12(8):1395-404. PMID:15296733
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